한빛사논문, 상위피인용논문
Abstract
Santiago Alejandro,1,5,* Yuree Lee,2,5 Takayuki Tohge,3,5,* Damien Sudre,1,5,6 Sonia Osorio,3 Jiyoung Park,4 Lucien Bovet,1,7 Youngsook Lee,4 Niko Geldner,2 Alisdair R. Fernie,3 and Enrico Martinoia1,4
1Institute of Plant Biology, University of Zurich, 8008 Zurich, Switzerland
2Department of Plant Molecular Biology, University of Lausanne, Quartier Sorge, 1015 Lausanne, Switzerland
3Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476 Potsdam, Germany
4POSTECH-UZH Cooperative Laboratory, Department of Integrative Bioscience and Biotechnology, Pohang University of Science and Technology, Pohang 790-784, South Korea
5These authors contributed equally to this work
6Present address: Biochimie et Physiologie Moleculaire des Plantes, CNRS, INRA, Universite?Montpellier 2, SupAgro. Bat 7, 2 Place Viala, 34060 Montpellier Cedex 1, France
7Present address: Philip Morris Products SA, PMI R&D, 2000 Neuchâtel, Switzerland
*Correspondence: Santiago Alejandro., Takayuki Tohge.
Summary
Lignin is the defining constituent of wood and the second most abundant natural polymer on earth. Lignin is produced by the oxidative coupling of three monolignols: p-coumaryl alcohol, coniferyl alcohol, and sinapyl alcohol [1]. Monolignols are synthesized via the phenylpropanoid pathway and eventually polymerized in the cell wall by peroxidases and laccases. However, the mechanism whereby monolignols are transported from the cytosol to the cell wall has remained elusive. Here we report the discovery that AtABCG29, an ATP-binding cassette transporter, acts as a p-coumaryl alcohol transporter. Expression of AtABCG29 promoter-driven reporter genes and a Citrine-AtABCG29 fusion construct revealed that AtABCG29 is targeted to the plasma membrane of the root endodermis and vascular tissue. Moreover, yeasts expressing AtABCG29 exhibited an increased tolerance to p-coumaryl alcohol by excreting this monolignol. Vesicles isolated from yeasts expressing AtABCG29 exhibited a p-coumaryl alcohol transport activity. Loss-of-function Arabidopsis mutants contained less lignin subunits and were more sensitive to p-coumaryl alcohol. Changes in secondary metabolite profiles in abcg29 underline the importance of regulating p-coumaryl alcohol levels in the cytosol. This is the first identification of a monolignol transporter, closing a crucial gap in our understanding of lignin biosynthesis, which could open new directions for lignin engineering.
논문정보
관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기