남진우 (Massachusetts Institute of Technology) | 한빛사논문 > 한빛사

한빛사논문, 상위피인용논문

조회3,994

Massachusetts Institute of Technology

CV File 385 kb

Genome Res., Published online before print, June 15, 2012 | doi: 10.1101/gr.140475.112 Long non-coding RNAs in C. elegans

Abstract

Jin-Wu Nam^1,2,3 and David Bartel^1,2,3,*

1Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
²Howard Hughes Medical Institute
³Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

* Corresponding author : David Bartel

Abstract
Thousands of long non-coding RNAs (lncRNAs) have been found in vertebrate animals, a few of which have known biological roles. To better understand the genomics and features of lncRNAs in invertebrates, we used available RNA-seq, poly(A)-site, and ribosome-mapping data to identify lncRNAs of C. elegans. We found hundreds of long intervening ncRNAs (lincRNAs), which had single- or multi-exonic structures that did not overlap protein-coding transcripts, and about seventy antisense lncRNAs (ancRNAs), which were complementary to protein-coding transcripts. Compared to protein-coding genes, the lncRNA genes tended to be expressed in stage-dependent manner. Approximately 30% of the newly identified lincRNAs showed little signal for sequence conservation and mapped antisense to clusters of 22G or 26G endogenous siRNAs, as would be expected if they serve as templates and targets for these siRNAs. The other 70% tended to be more conserved and included lincRNAs with intriguing expression and sequence features associating them with processes such as dauer formation, male identity, sperm formation, and interaction with sperm-specific mRNAs. Our study provides a glimpse into the lncRNA content of a non-vertebrate animal and a resource for future studies of lncRNA function.

논문정보

형식Research article
게재일2012년 06월 (BRIC 등록일 )
연구진국외 연구진
분야 생명과학 > 유전학
피인용횟수120회 이상 인용된 논문 (상위피인용논문 등록일 2019-04-11)

댓글 작성 로그인

CV 열람하기

연락처 보기