Seong Woon Roh1, Guy C.J. Abell2, Kyoung-Ho Kim1, Young-Do Nam1, Jin-Woo Bae1,*
1 Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, HoeGi-Dong 1, DongDaeMun-Gu, Seoul 130-701, Republic of Korea
2 CSIRO, Marine and Atmospheric Research and Wealth from Oceans, National Research Flagship, Hobart, Tasmania, Australia
Recent advances in molecular biology have resulted in the application of DNA microarrays and next-generation sequencing (NGS) technologies to the field of microbial ecology. This review aims to examine the strengths and weaknesses of each of the methodologies, including depth and ease of analysis, throughput and cost-effectiveness. It also intends to highlight the optimal application of each of the individual technologies toward the study of a particular environment and identify potential synergies between the two main technologies, whereby both sample number and coverage can be maximized. We suggest that the efficient use of microarray and NGS technologies will allow researchers to advance the field of microbial ecology, and importantly, improve our understanding of the role of microorganisms in their various environments.