한빛사논문
- 조회2,761
Abstract
Won-Yong Songa,b,c, Kwan-Sam Choic, De Angeli Alexisa, Enrico Martinoiaa,b,1,2, and Youngsook Leeb,1,2
aInstitute of Plant Biology, University of Zurich, CH-8008 Zurich, Switzerland;
bPohang University of Science and Technology-University of Zurich Cooperative Laboratory, Department of Integrative Bioscience and Biotechnology, World Class University Program, Pohang University of Science and Technology, Pohang 790-784, Korea; and
cDivision of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea
Edited by Michael G. Palmgren, Centre for Membrane Pumps in Cells and Disease, Danish National Research Foundation, University of Copenhagen, Frederiksberg C, Denmark, and accepted by the Editorial Board October 14, 2011 (received for review March 28, 2011)
Abstract
Calcium (Ca) is an important structural component of plant cell walls and an intracellular messenger in plants and animals. Therefore, plants tightly control the balance of Ca by regulating Ca uptake and its transfer from cell to cell and organ to organ. Here, we propose that Brassica juncea PCR1 (PCR1), a member of the plant cadmium resistance (PCR) protein family in Indian mustard, is a Ca2+ efflux transporter that is required for the efficient radial transfer of Ca2+ in the root and is implicated in the translocation of Ca to the shoot. Knock-down lines of BjPCR1 were greatly stunted and translocated less Ca to the shoot than did the corresponding WT. The localization of BjPCR1 to the plasma membrane and the preferential expression of BjPCR1 in the root epidermal cells of WT plants suggest that BjPCR1 antisense plants could not efficiently transfer Ca2+ from the root epidermis to the cells located inside the root. Protoplasts isolated from BjPCR1 antisense lines had lower Ca2+ efflux activity than did those of the WT, and membrane vesicles isolated from BjPCR1-expressing yeast exhibited increased Ca2+ transport activity. Inhibitor studies, together with theoretical considerations, indicate that BjPCR1 exports one Ca2+ in exchange for three protons. Root hair-specific expression of BjPCR1 in Arabidopsis results in plants that exhibit increased Ca2+ resistance and translocation. In conclusion, our data support the hypothesis that BjPCR1 is an exporter required for the translocation of Ca2+ from the root epidermis to the inner cells, and ultimately to the shoot.
calcium translocationplasma membrane H+/Ca2+ antiporternutritioncalcium homeostasis
Footnotes
1E.M. and Y.L. contributed equally to this work.
2To whom correspondence may be addressed.
Author contributions: W.-Y.S., K.-S.C., E.M., and Y.L. designed research; W.-Y.S. performed research; W.-Y.S., K.-S.C., D.A.A., E.M., and Y.L. analyzed data; and W.-Y.S., D.A.A., E.M., and Y.L. wrote the paper.
논문정보
- Research article
- 2011년 11월 (BRIC 등록일 )
- 국내(교신)+국외 연구진
- 생명과학 > 식물학
관련 보도자료
- Bio통신원
- 2011. 11. 24
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