한빛사논문, 상위피인용논문
- 조회4,956
Abstract
, Dalia M. Dhingra *, Theo Odijk 
, Juan J. de Pablo 
, Michael D. Graham , Rod Runnheim *, Dan Forrest *, and David C. Schwartz *¶
*Laboratory for Molecular and Computational Genomics, Laboratory of Genetics, and Biotechnology Center, University of Wisconsin, 425 Henry Mall, Madison, WI 53706; Department of Chemistry, University of Wisconsin, 1101 University Avenue, Madison, WI 53706; Complex Fluids Theory, Faculty of Applied Sciences, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands; and Department of Chemical and Biological Engineering, University of Wisconsin, 1415 Engineering Drive, Madison, WI 53706
Communicated by David E. Housman, Massachusetts Institute of Technology, Cambridge, MA, December 14, 2006 (received for review November 12, 2006)
Molecular confinement offers new routes for arraying large DNA molecules, enabling single-molecule schemes aimed at the acquisition of sequence information. Such schemes can rapidly advance to become platforms capable of genome analysis if elements of a nascent system can be integrated at an early stage of development. Integrated strategies are needed for surmounting the stringent experimental requirements of nanoscale devices regarding fabrication, sample loading, biochemical labeling, and detection. We demonstrate that disposable devices featuring both micro- and nanoscale features can greatly elongate DNA molecules when buffer conditions are controlled to alter DNA stiffness. Furthermore, we present analytical calculations that describe this elongation. We also developed a complementary enzymatic labeling scheme that tags specific sequences on elongated molecules within described nanoslit devices that are imaged via fluorescence resonance energy transfer. Collectively, these developments enable scaleable molecular confinement approaches for genome analysis.
Author contributions: K.J., D.M.D., and D.C.S. designed research; K.J. and D.M.D. performed research; K.J., T.O., R.R., and D.F. contributed new reagents/analytic tools; K.J., D.M.D., T.O., J.J.d.P., M.D.G., and D.C.S. analyzed data; and K.J., D.M.D., T.O., and D.C.S. wrote the paper.
The authors declare no conflict of interest.
¶To whom correspondence should be sent at the * address.
논문정보
- Research article
- 2007년 02월 (BRIC 등록일 )
- 국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
- 60회 이상 인용된 논문 (상위피인용논문 등록일 2011-05-01)
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기
