한빛사논문, 상위피인용논문
- 조회7,918
Abstract
Suk-Jin Haa,b,1, Jonathan M. Galazkac,1, Soo Rin Kima,b, Jin-Ho Choia,b, Xiaomin Yangd, Jin-Ho Seoe, N. Louise Glassf, Jamie H. D. Catec,g,2, and Yong-Su Jina,b,2
aDepartment of Food Science and Human Nutrition, University of Illinois, Urbana, IL 61801;
bInstitute for Genomic Biology, University of Illinois, Urbana, IL 61801;
cDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720;
dBP Biofuels Business Unit, Berkeley, CA 97420;
eDepartment of Agricultural Biotechnology, Seoul National University, Seoul 152-742, Korea;
fDepartment of Plant and Microbial Biology, University of California, Berkeley, CA 94720; and
gPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720
Edited by Lonnie O'Neal Ingram, University of Florida, Gainesville, FL, and approved December 1, 2010 (received for review July 22, 2010)
1S.-J.H. and J.M.G. contributed equally to this work.
Abstract
The use of plant biomass for biofuel production will require efficient utilization of the sugars in lignocellulose, primarily glucose and xylose. However, strains of Saccharomyces cerevisiae presently used in bioethanol production ferment glucose but not xylose. Yeasts engineered to ferment xylose do so slowly, and cannot utilize xylose until glucose is completely consumed. To overcome these bottlenecks, we engineered yeasts to coferment mixtures of xylose and cellobiose. In these yeast strains, hydrolysis of cellobiose takes place inside yeast cells through the action of an intracellular β-glucosidase following import by a high-affinity cellodextrin transporter. Intracellular hydrolysis of cellobiose minimizes glucose repression of xylose fermentation allowing coconsumption of cellobiose and xylose. The resulting yeast strains, cofermented cellobiose and xylose simultaneously and exhibited improved ethanol yield when compared to fermentation with either cellobiose or xylose as sole carbon sources. We also observed improved yields and productivities from cofermentation experiments performed with simulated cellulosic hydrolyzates, suggesting this is a promising cofermentation strategy for cellulosic biofuel production. The successful integration of cellobiose and xylose fermentation pathways in yeast is a critical step towards enabling economic biofuel production.
biofuels, cellodextrin transporter, cofermentation, intracellular β-glucosidase
Footnotes
2To whom correspondence may be addressed.
Author contributions: S.-J.H., J.M.G., X.Y., J.H.D.C., and Y.-S.J. designed research; S.-J.H., S.R.K., J.-H.C., and Y.-S.J. performed research; J.-H.S. and N.L.G. contributed new reagents/analytic tools; S.-J.H., J.M.G., X.Y., J.H.D.C., and Y.-S.J. analyzed data; and S.-J.H., J.M.G., X.Y., J.H.D.C., and Y.-S.J. wrote the paper.
The authors declare no conflict of interest.
This article is a PNAS Direct Submission.
This article contains supporting information online at
www.pnas.org/lookup/suppl/doi:10.1073/pnas.1010456108/-/DCSupplemental.
논문정보
- Research article
- 2010년 12월 (BRIC 등록일 )
- 국내+국외 연구진
- 생명과학 > 생물공학
- 60회 이상 인용된 논문 (상위피인용논문 등록일 2013-11-28)
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