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J. Immunol. Methods, doi:10.1016/j.jim.2010.09.033 A novel method for procuring a large quantity of mature murine eosinophils in vivo

Abstract

Wonyoung Kim^a, Juyang Kim^b, Changshik Shin^c, Hyunju Kim^a, Younkyung Doh^c, Hong R. Cho^b and Byungsuk Kwon^{a, b}

^a School of Biological Sciences, University of Ulsan, Ulsan, Republic of Korea
^b Biomedical Research Center, Ulsan University Hospital and School of Medicine, University of Ulsan, Republic of Korea
^c School of Nanobiotechnology and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea

Received 19 April 2010; revised 14 September 2010; accepted 23 September 2010. Available online 30 September 2010.

Abstract
Eosinophils are rare hematopietic cells that normally constitute only 1 ~ 3% of peripheral blood leukocytes. It would be of help for the purpose of research to obtain a large quantity of eosinophils. In this study, we wanted to develop a novel strategy to induce massive expansion of murine eosinophils in vivo, based on the observation showing that treatment of IL-33 induces eosinophilia in mice. We generated an EL-4 lymphoma cell line (herein named EL-4-IL-33) that was engineered to secrete an active form of IL-33. We found that Siglec-F⁺ granulocyte numbers increased by 1850-fold in the peritoneal cavity 10 days after inoculation with 1 × 10⁷ EL-4-IL-33 cells. This number corresponds to 74-fold increase, as compared with the number of Siglec-F⁺ granulocytes in mice that received wild-type EL-4 cells. Siglec-F⁺ granulocytes expanded by IL-33 had the circular nucleus and expressed eosinophil-specific genes. They also showed some functional characteristics of eosinophils in that they had the ability to respond to IL-5 for survival and eotaxin-1 for chemoattaxis and to produce bioactive eosinophil peroxidase, suggesting that these cells are genuine eosinophils. Our results indicate that sustained secretion of IL-33 by lymphoma cells in the peritoneal cavity is highly effective in increasing peritoneal eosinophil numbers. Therefore, our simple method to obtain eosinphils on a large scale might be of value for eosinophil studies.

Keywords: Eosinophil; Interleukin-33; Eosinophilia

논문정보

형식Research article
게재일2010년 10월 (BRIC 등록일 )
연구진국내 연구진
분야 생명과학 > 면역학
구분구・추천논문

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