한빛사논문
- 조회2,467
Abstract
Kidong Parka,d, Larry J. Milleta,d, Namjung Kimc, Huan Lic, Xiaozhong Jinc, Gabriel Popescua,d,e, N. R. Aluruc,d,e, K. Jimmy Hsiab,c,d, and Rashid Bashira,b,d,1
aDepartment of Electrical and Computer Engineering,
bDepartment of Bioengineering,
cDepartment of Mechanical Science and Engineering,
dMicro and Nanotechnology Laboratory, and
eBeckman Institute of Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801
Edited* by Karl Hess, Beckman Institute, Urbana, IL, and approved October 5, 2010 (received for review August 3, 2010)
Abstract
The characterization of physical properties of cells such as their mass and stiffness has been of great interest and can have profound implications in cell biology, tissue engineering, cancer, and disease research. For example, the direct dependence of cell growth rate on cell mass for individual adherent human cells can elucidate the mechanisms underlying cell cycle progression. Here we develop an array of micro-electro-mechanical systems (MEMS) resonant mass sensors that can be used to directly measure the biophysical properties, mass, and growth rate of single adherent cells. Unlike conventional cantilever mass sensors, our sensors retain a uniform mass sensitivity over the cell attachment surface. By measuring the frequency shift of the mass sensors with growing (soft) cells and fixed (stiff) cells, and through analytical modeling, we derive the Young’s modulus of the unfixed cell and unravel the dependence of the cell mass measurement on cell stiffness. Finally, we grew individual cells on the mass sensors and measured their mass for 50+ hours. Our results demonstrate that adherent human colon epithelial cells have increased growth rates with a larger cell mass, and the average growth rate increases linearly with the cell mass, at 3.25%/hr. Our sensitive mass sensors with a position-independent mass sensitivity can be coupled with microscopy for simultaneous monitoring of cell growth and status, and provide an ideal method to study cell growth, cell cycle progression, differentiation, and apoptosis.
cell mechanics, cell division, bio-sensor
Footnotes
1To whom correspondence should be addressed.
Author contributions: K.P., L.J.M., G.P., N.R.A., K.J.H., and R.B. designed research; K.P., L.J.M., N.K., H.L., X.J., and K.J.H. performed research; K.P., N.K., H.L., X.J., N.R.A., K.J.H., and R.B. contributed new reagents/analytic tools; K.P., L.J.M., N.K., H.L., X.J., N.R.A., K.J.H., and R.B. analyzed data; and K.P., L.J.M., and R.B. wrote the paper.
The authors declare no conflict of interest.
*This Direct Submission article had a prearranged editor.
This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1011365107/-/DCSupplemental.
논문정보
- Research article
- 2010년 11월 (BRIC 등록일 )
- 국외 연구진
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