한빛사논문
- 조회6,085
Abstract
Sun-Yang Park,1,3,4 Michael J. Cromie,1,2,3,5 Eun-Jin Lee,1,2,5 and Eduardo A. Groisman1,2,5,*
1Department of Molecular Microbiology
2Howard Hughes Medical Institute
Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8230, St. Louis, MO 63110, USA
3These authors contributed equally to this work
4Present address: Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine,295 Congress Avenue, New Haven, CT 06536-0812, USA
5Present address: Section of Microbial Pathogenesis, Howard Hughes Medical Institute, Yale University School of Medicine,Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, CT 06536-0812, USA
*Correspondence
SUMMARY
Bacterial mRNAs often contain leader sequences that respond to specific metabolites or ions by altering expression of the associated downstream protein-coding sequences. Here we report that the leader RNA of the Mg2+ transporter gene mgtA of Salmonella enterica, which was previously known to function as a Mg2+-sensing riboswitch, harbors an 18 codon proline-rich open reading frame. termed mgtL.that permits intracellular proline to regulate mgtA expression. Interfering with mgtL translation by genetic, pharmacological, or environmental means was observed to increase the mRNA levels from the mgtA coding region. Substitution of the mgtL proline codons by other codons abolished the response to proline and to hyperosmotic stress but not to Mg2+. Our findings show that mRNA leader sequences can consist of complex regulatory elements that utilize different mechanisms to sense separate signals and mediate an appropriate cellular response.
논문정보
- Research article
- 2010년 09월 (BRIC 등록일 )
- 국외 연구진
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