한빛사논문
- 조회1,865
Abstract
Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 68-233, Cambridge, MA 02139-4307
Contributed by Alexander Rich, July 21, 2005
The vaccinia virus (VV) E3L protein is essential for virulence and
has anti-apoptotic activity. In mice, Z-DNA-binding activity of the
N-terminal domain of E3L (Z![{alpha}](\"http://www.pnas.org/math/agr.gif\")
) is
necessary for viral lethality. Here, we report that inhibition of
hygromycin-B-induced apoptosis in HeLa cells depends on Z-DNA binding
of the E3L Z domain. Z-DNA-binding
domains of other proteins are equally effective in blocking
apoptosis. Using a transient reporter assay, we demonstrate
transactivation of human IL-6, nuclear factor of activated T
cells (NF-AT), and p53 genes by E3L. This activation also
requires Z-DNA binding of the N-terminal domain of E3L. Overall,
this work suggests that the important role of E3L in VV
pathogenesis involves modulating expression of host cellular genes at
the transcriptional level and inhibiting apoptosis of host cells
through Z-DNA binding.
Author contributions: J.-A.K. and A.R. designed research; J.-A.K. performed research; J.-A.K. and A.R. analyzed data; and J.-A.K. and A.R. wrote the paper.
*To whom correspondence may be addressed. Fax: 617-253-8699 (A.R.) or
Jin-Ah Kwon.논문정보
- Research article
- 2005년 08월 (BRIC 등록일 )
- 국외 연구진
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