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Proc. Natl. Acad. Sci. USA, October 1, 2026 123 (40) e2622107123 | https://doi.org/10.1073/pnas.2622107123 Signaling by a tyrosine-sulfated peptide balances growth and osmotic stress response in rice

Yejin Shim^a, Ellen Y. Rim^b, Jin C.-Y. Liao^a, Myeong-Je Cho^c, George Austin^c, Patrick W. Carlos^d,e, Sameer S. Kulkarni^d,e, Richard J. Payne^d,e, Maria Florencia Ercoli^a,1,2, and Pamela C. Ronald^a,f,1

^aDepartment of Plant Pathology, University of California, Davis, CA95616;
^bDepartment of Chemical Engineering, Stanford University, CA 94305;
^cInnovative Genomics Institute, University of California, Berkeley, CA 94720;
^dSchool of Chemistry, TheUniversity of Sydney, Sydney, NSW 2006, Australia;
^eAustralian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Sydney, Sydney, NSW 2006, Australia; and
^fThe Genome Center, University of California, Davis, CA 95616

¹To whom correspondence may be addressed.
²Present address: Department of Botany and PlantSciences, University of California, Riverside, Riverside,CA 92521.

Abstract

Peptide hormone signaling coordinates plant growth and osmotic stress responses, yet how the transition between these responses is regulated remains poorly understood. Here, we investigated the function of the rice PLANT PEPTIDES CONTAINING SULFATED TYROSINE 8 (OsPSY8) peptide in osmotic stress responses. OsPSY8 was predominantly expressed in root tissues under nonstress conditions, with preferential expression in lateral roots where it promoted root growth. Osmotic stress rapidly reduced OsPSY8 expression in roots through the OsWRKY24 transcription factor. Loss-of-function ospsy8 mutants exhibited enhanced osmotic stress tolerance, whereas OsPSY8 overexpression increased osmotic stress susceptibility. Transcriptomic analyses revealed that disruption of OsPSY8 activated stress-responsive pathways, including those associated with lignin biosynthesis, compatible solute production, cell wall remodeling, and reactive oxygen species scavenging, and was accompanied by increased lignin accumulation in roots. In contrast, overexpression of OsPSY8 resulted in maintenance of growth-associated transcriptional programs while suppressing stress-responsive pathways under osmotic stress. Together, these findings identify OsPSY8 as an important regulator of the transition from growth to stress adaptation in rice and suggest that stress-induced repression of PSY signaling is required to disengage growth programs and activate adaptive responses during osmotic stress.

논문소개전체 설명

식물은 가뭄과 같은 스트레스에 노출되면 생장을 줄이고 생존을 위한 적응 반응으로 전환해야 합니다. 본 연구에서는 벼의 펩타이드 호르몬 OsPSY8이 이러한 생장과 스트레스 적응 사이의 균형을 조절한다는 사실을 밝혔습니다.

정상조건에서 OsPSY8은 주로 뿌리에서 발현되어 생장을 촉진하지만, 삼투 스트레스가 발생하면 전사인자 OsWRKY24에 의해 그 발현이 감소합니다. ospsy8 돌연변이체는 스트레스 저항성이 증가한 반면, OsPSY8 과발현 식물은 정상 조건에서 뿌리가 길어진 대신 스트레스에 더 민감했습니다. 또한 ospsy8에서는 리그닌 생합성, 활성산소 제거 등 다양한 스트레스 적응 경로가 활성화되었습니다.

이 결과는 OsPSY8 신호의 감소가 벼를 ‘생장 모드’에서 ‘스트레스 적응 모드’로 전환시키는 중요한 조절 기작임을 보여줍니다. 향후 이 신호를 정밀하게 조절한다면 생장 저하를 최소화하면서 환경 스트레스에 강한 작물을 개발하는 데 활용할 수 있을 것으로 기대합니다.

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