Alexander S. Bates^1,2,*,**, Jasper S. Phelps^1,3,*,**, Minsu Kim^1,4,5,*, Helen H. Yang^1,*, Arie Matsliah⁶, Zaki Ajabi¹, Eric Perlman⁷, Kevin M. Delgado¹, Mohammed Abdal Monium Osman¹, Christopher K. Salmon⁶, Jay Gager⁶, Benjamin Silverman⁶, Sophia Renauld¹, Farzaan Salman^8,9, Janki Patel¹, Matthew F. Collie¹, Jingxuan Fan¹, Diego A. Pacheco¹, Yunzhi Zhao¹, Wenyi Zhang¹, Laia Serratosa Capdevila¹⁰, Ruairí J.V. Roberts¹⁰, Eva J. Munnelly¹⁰, Nina Griggs¹⁰, Helen Langley¹⁰, Borja Moya-Llamas¹⁰, Zuoyu Zhang^11,12, Ryan T. Maloney^13,14,15, Szi-chieh Yu⁶, Amy R. Sterling⁶, Marissa Sorek⁶, Krzysztof Kruk¹⁶, Nikitas Serafetinidis¹⁶, Serene Dhawan⁶, Finja Klemm¹⁷, Paul Brooks¹⁸, Ellen Lesser¹⁹, Jessica M. Jones²⁰, Sara E. Pierce-Lundgren²⁰, Su-Yee Lee²⁰, Yichen Luo²⁰, Andrew P. Cook²¹, Theresa H. McKim²², Dimitrios Stasi Giakoumas^23,24, Benjamin Gorko²⁵, Justin Ellis-Joyce^26,27, Jiayi Zhang²⁸, Emily C. Kophs²⁹, Tjalda Falt³⁰, Alexa M. Negron-Morales³¹, Austin Burke⁶, James Hebditch⁶, Kyle P. Willie⁶, Ryan Willie⁶, Sergiy Popovych³², Nico Kemnitz³², Dodam Ih³², Kisuk Lee³², Ran Lu³², Akhilesh Halageri³², J. Alexander Bae³², Ben Jourdan³³, Gregory Schwartzman³⁴, Damian D. Demarest³⁵, Emily Behnke⁶, Doug Bland¹⁶, Anne Kristiansen¹⁶, Jaime Skelton¹⁶, Tom Stocks¹⁶, Dustin Garner²⁵, Anthony Hernandez¹⁶, Sandeep Kumar⁶, The BANC-FlyWire Consortium^, Kevin C. Daly^8,36, Sven Dorkenwald³⁷, Forrest Collman³⁷, Marie P. Suver²⁹, Lisa M. Fenk³⁰, Michael J. Pankratz³⁵, Zepeng Yao^23,38,39,40, Fei Wang²⁸, Stephen J. Huston⁴¹, Tomke Stürner⁴², Gregory S.X.E. Jefferis⁴², Katharina Eichler¹⁷, Andrew M. Seeds³¹, Stefanie Hampel³¹, Sweta Agrawal²¹, Tatsuo S. Okubo^11,12, Meet Zandawala^43,44, Thomas Macrina³², Diane-Yayra Adjavon²⁶, Jan Funke²⁶, John C. Tuthill²⁰, Anthony Azevedo²⁰, H. Sebastian Seung^6,45, Benjamin L. de Bivort^13,14, Mala Murthy^6,**, Jan Drugowitsch^1,**, Rachel I. Wilson^1,**, Wei-Chung Allen Lee^1,46,**
 
1Department of Neurobiology, Harvard Medical School, Boston, MA, USA
2Centre for Neural Circuit and Behaviour, University of Oxford, Oxford, UK
3Present address: Neuroengineering Laboratory, Brain Mind Institute and Institute of  Bioengineering, EPFL, Lausanne, Switzerland
4Present address: Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA
5Present address: Center for Brain Science, Harvard University, Cambridge, MA, USA
6Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA
7Yikes LLC, Baltimore, MD, USA
8Department of Biology, West Virginia University, Morgantown, WV, USA  
9Present address: Department of Neurobiology, Harvard Medical School, Boston, MA, USA
10Aelysia LTD, Bristol, UK  
11Beijing Institute for Brain Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China  
12Chinese Institute for Brain Research, Beijing, Beijing, China
13Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA
14Center for Brain Science, Harvard University, Cambridge, MA, USA
15Present address: Psychology Department, Colorado College, Colorado Springs, CO, USA  
16Eyewire, Boston, MA, USA
17Genetics Department, Leipzig University, Leipzig, Germany
18Zoology Department, University of Cambridge, Cambridge, UK
19Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL, USA
20Department of Neurobiology and Biophysics, University of Washington, Seattle, WA, USA
21School of Neuroscience, Virginia Tech, Blacksburg, VA, USA
22Department of Biology, University of Nevada Reno, Reno, NV, USA
23Department of Biology, University of Florida, Gainesville, FL, USA
24Present address: Interdisciplinary Graduate Program in Neuroscience, University of Iowa, Iowa City, IA, USA
25Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara,
Santa Barbara, CA, USA
26HHMI Janelia, Ashburn, VA, USA  
27Department of Neuroscience, Johns Hopkins University, Baltimore, MD, USA
28Institute of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China
29Department of Biological Sciences, Vanderbilt University, Nashville, TN, USA
30Max Planck Institute for Biological Intelligence, Martinsried, Germany  
31Institute of Neurobiology, University of Puerto Rico Medical Sciences Campus, San Juan,Puerto Rico
32Zetta AI LLC, Sherrill, NY, USA
33School of Informatics, University of Edinburgh, Edinburgh, UK
34Japan Advanced Institute of Science and Technology (JAIST), Nomi, Japan  
35Molecular Brain Physiology and Behavior, LIMES Institute, University of Bonn, Bonn, Germany
36Department of Neuroscience, West Virginia University, Morgantown, WV, USA
37Allen Institute for Brain Science, Seattle, WA, USA
38Florida Chemical Senses Institute, University of Florida, Gainesville, FL, USA
39McKnight Brain Institute, University of Florida, Gainesville, FL, USA
40Genetics Institute, University of Florida, Gainesville, FL, USA  
41Department of Neuroscience, Zuckerman Mind Brain Behavior Institute, Columbia  University, New York, NY, USA
42Neurobiology Division, MRC Laboratory of Molecular Biology, Cambridge, UK
43Department of Biochemistry and Molecular Biology, University of Nevada Reno, Reno, NV, USA
44Neurobiology and Genetics, Theodor-Boveri-Institute, Biocenter, Julius-Maximilians-University of Würzburg, Am Hubland, Würzburg, Germany.
45Computer Science Department, Princeton University, Princeton, NJ, USA
46F.M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA

*These authors contributed equally.
**Correspondence to Alexander S. Bates, Jasper S. Phelps, Mala Murthy, Jan Drugowitsch, Rachel I. Wilson or Wei-Chung Allen Lee.