Hydrogel-based tissue adhesives hold promise for diverse biomedical applications, yet achieving strong, durable adhesion to wet, dynamic biological surfaces remains challenging. The catechol containing amino acid 3,4-dihydroxy-L phenylalanine (DOPA) is a crucial functional motif in mussel-inspired adhesives and has been extensively investigated for incorporation into various biomaterials. However, conventional approaches of DOPA incorporation and subsequent crosslinking have shown limited potential for clinical translation. We developed a bioinspired strategy involving pH controlled enzymatic conversion of tyrosine residues in porcine gelatin into DOPA. The resulting catechol-functionalized gelatin was blended with native tyrosine-rich gelatin to form a hybrid adhesive hydrogel, termed DOPA–Tyrosine Gelatin (DTG) bioglue. DTG was designed to achieve (1) rapid visible light–driven Rutheniumbased crosslinking for structural integrity and (2) enhanced interfacial adhesion via DOPAmediated interactions with tissue surface. Its shear-thinning and fast-curing properties enable use as a bioink for both extrusion-based and digital light processing (DLP) 3D bioprinting. We demonstrated the DTG assisted tissue–tissue integration for large-area construct assembly, addressing current volumetric fabrication limitations in micro-extrusion bioprinting. Moreover, it demonstrated superior hemostatic performance in rat liver and vascular injury models. Collectively, DTG bioglue is a promising candidate for next generation adhesive biomaterial for regenerative medicine and surgical applications.