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상위피인용논문

조회42

홍정일Jungil Hong

서울여자대학교

LWT-Food Sci. Technol., Volume 153, January 2022, 112565 | https://doi.org/10.1016/j.lwt.2021.112565 Application of the MTT-based colorimetric method for evaluating bacterial growth using different solvent systems

Yeong Ji Oh ^a, Jungil Hong ^{b *}

^aDepartment of Food and Animal Biotechnology, Research Institute for Agriculture and Life Sciences, Center for Food and Bioconvergence, Seoul National University, Seoul, 08826, Republic of Korea
^bDivision of Applied Food System, College of Natural Science, Seoul Women's University, 621 Hwarangro, Nowon-gu, Seoul, 01797, Republic of Korea

^*Corresponding author: correspondence to Jungil Hong

Abstract

Although the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is widely used to measure the growth of animal cells, several interfering factors and obstacles for analyzing bacterial growth and for solubilizing bacterial-formazan have been reported. In this study, growth of Gram-negative Escherichia coli and Gram-positive Leuconostoc mesenteroides were analyzed using the MTT assay, and different solvents, specifically dimethyl sulfoxide, 1% Triton X-100, 1% Bile bovine, 10% sodium dodecyl sulfate, and sodium hydroxide (NaOH) were evaluated for solubilizing bacterial-formazan. MTT formazan that had been reduced by L. mesenteroides had fully developed color within 15 min in all the solvents tested. The solvent with the highest peak absorbance was 1 N NaOH. MTT formazan did not have a fully developed color response in the 0.001–0.1 N NaOH range, and its color intensity was not further enhanced at concentrations higher than 1 N. For all solvents, the color intensity of dissolved formazan was significantly higher in E. coli than in L. mesenteroides. The MTT assay showed sufficient sensitivity to detect bacterial cells during early growth phase that could not be detected by the plate counting method. These results suggest that NaOH (1 N) is a suitable solvent for analyzing bacterial growth in MTT assays.

논문정보

형식Research article
게재일2022년 01월 (BRIC 등록일 2025-01-03)
연구진국내 연구진
분야
피인용횟수60회 이상 인용된 논문

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