이진우 (연세대학교 의과대학) | 상위피인용논문 > 한빛사

상위피인용논문

조회488

연세대학교 의과대학

CV File 350 kb

Acta Biomater., Volume 38, 1 July 2016 | https://doi.org/10.1016/j.actbio.2016.04.030 Cell recruiting chemokine-loaded sprayable gelatin hydrogel dressings for diabetic wound healing

Dong Suk Yoon ^{a 1}, Yunki Lee ^{b 1}, Hyun Aae Ryu ^a, Yeonsue Jang ^a, Kyoung-Mi Lee ^{a d}, Yoorim Choi ^{a d}, Woo Jin Choi ^a, Moses Lee ^a, Kyung Min Park ^c, Ki Dong Park ^b, Jin Woo Lee ^{a d}

^aDepartment of Orthopaedic Surgery, Yonsei University College of Medicine, Seoul 120-752, South Korea
^bDepartment of Molecular Science and Technology, Ajou University, Suwon 443-749, South Korea
^cDivision of Bioengineering, Incheon National University, Incheon 406-772, South Korea
^dBrain Korea 21 PLUS Project for Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, South Korea

¹These authors equally contributed to this study.
Corresponding author: correspondence to Jin Woo Lee

Abstract

In this study, we developed horseradish peroxidase (HRP)-catalyzed sprayable gelatin hydrogels (GH) as a bioactive wound dressing that can deliver cell-attracting chemotactic cytokines to the injured tissues for diabetic wound healing. We hypothesized that topical administration of chemokines using GH hydrogels might improve wound healing by inducing recruitment of the endogenous cells. Two types of chemokines (interleukin-8; IL-8, macrophage inflammatory protein-3α; MIP-3α) were simply loaded into GH hydrogels during in situ cross-linking, and then their wound-healing effects were evaluated in streptozotocin-induced diabetic mice. The incorporation of chemokines did not affect hydrogels properties including swelling ratio and mechanical stiffness, and the bioactivities of IL-8 and MIP-3α released from hydrogel matrices were stably maintained. In vivo transplantation of chemokine-loaded GH hydrogels facilitated cell infiltration into the wound area, and promoted wound healing with enhanced re-epithelialization/neovascularization and increased collagen deposition, compared with no treatment or the GH hydrogel alone. Based on our results, we suggest that cell-recruiting chemokine-loaded GH hydrogel dressing can serve as a delivery platform of various therapeutic proteins for wound healing applications.

논문정보

형식Research article
게재일2016년 07월 (BRIC 등록일 2023-06-09)
연구진국내 연구진
분야
피인용횟수120회 이상 인용된 논문

댓글 작성 로그인

CV 열람하기

연락처 보기