상위피인용논문
- 조회1,149
Abdullah-Al-Nahain,† Jung-Eun Lee,‡ Insik In,§ Haeshin Lee,∥ Kang Dae Lee,⊥ Ji Hoon Jeong,*,‡ and Sung Young Park*,@
† Department of Green Bio Engineering, Korea National University of Transportation, Chungju 380-702, Republic of Korea
‡ School of Pharmacy, Sungkyunkwan University, 300 Cheoncheon-dong, Jangan-gu, Suwon, Gyeonggi-do 440-746, Republic of Korea
§ Department of Polymer Science and Engineering, Korea National University of Transportation, Chungju 380-702, Republic of Korea
∥ Department of Chemistry, KAIST, Daejeon 305-701, Republic of Korea
⊥ Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Kosin University, Busan, Republic of Korea
@ Department of Chemical and Biological Engineering, Korea National University of Transportation, Chungju 380-702, Republic of Korea
*Corresponding Authors : Ji Hoon Jeong, Sung Young Park
Author Contributions
A.A.-N. and J.-E.L. contributed equally to this work.
Abstract
This work demonstrates the way to achieve efficient and target specific delivery of a graphene quantum dot (GQD) using hyaluronic acid (HA) (GQD-HA) as a targeting agent. HA has been anchored to a GQD that accepts the fascinating adhesive properties of the catechol moiety, dopamine hydrochloride, conjugated to HA, which was confirmed by X-ray photoelectron spectroscopy. Transmission electron microscopy revealed a particle size of ∼20 nm, and the fluorescence spectra revealed significant fluorescence intensity even after the anchoring of HA. The prepared GQD-HA was applied to CD44 receptor overexpressed tumor-bearing balb/c female mice, and the in vivo biodistribution investigation demonstrated more bright fluorescence from the tumor tissue. In vitro cellular imaging, via a confocal laser scanning microscope, exhibited strong fluorescence from CD44 overexpressed A549 cells. Both in vivo and in vitro results showed the effectiveness of using HA as targeting molecule. The loading and release kinetics of the hydrophobic drug doxorubicin from a GQD under mildly acidic conditions showed that a GQD can be considered as a novel drug carrier, while the nontoxic behavior from the MTT assay strongly supports the identification of GQD-HA as a biocompatible material.
KEYWORDS : graphene quantum dots, hyaluronic acid, cell imaging, target delivery, in vivo
논문정보
- Research article
- 2013년 09월 (BRIC 등록일 2020-02-13)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
- 120회 이상 인용된 논문
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