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상위피인용논문

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김준성Jun Sung Kim

바이테리얼즈

Toxicol. Vitro, Volume 27, Issue 4, June 2013, Pages 1187-1195 | https://doi.org/10.1016/j.tiv.2013.02.010 Zinc oxide nanoparticle induced autophagic cell death and mitochondrial damage via reactive oxygen species generation

Kyeong-Nam Yu^a,1, Tae-Jong Yoon^b,1, Arash Minai-Tehrani^a, Ji-Eun Kim^a,c, Soo Jin Park^d, Min Sook Jeong^d, Shin-Woo Ha^e, Jin-Kyu Lee^f, Jun Sung Kim^d,*, Myung-Haing Cho^a,c,g,h,*

^aLaboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea
^b Department of Applied BioScience, CHA University, Seoul 135-907, Republic of Korea
^cDepartment of Nano Fusion Technology, Graduate School of Convergence Science and Technology, Seoul National University, Suwon 443-270, Republic of Korea
^d R&D Center, Biterials Co., Ltd., Seoul 140-200, Republic of Korea
^eDivision of Endocrinology, Metabolism and Lipids, Emory University School of Medicine, 101 Woodruff Circle, WMRB Room 1327 Atlanta, GA 30322, United States
^fDepartment of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-742, Republic of Korea
^g Graduate Group of Tumor Biology, Seoul National University, Seoul 151-742, Republic of Korea
^hAdvanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, Republic of Korea

^*Corresponding authors : Jun Sung Kim, Myung-Haing Cho

¹These authors contributed equally.

Abstract

Zinc oxide nanoparticles (ZnO-np) are used in an increasing number of industrial products such as paint, coating and cosmetics, and in other biological applications. There have been many suggestions of a ZnO-np toxicity paradigm but the underlying molecular mechanisms about the toxicity of ZnO-np remain unclear. This study was done to determine the potential toxicity of ZnO-np and to assess the toxicity mechanism in normal skin cells. Synthesized ZnO-np generated reactive oxygen species (ROS), as determined by electron spin resonance. After uptake into cells, ZnO-np induced ROS in a concentration- and time-dependent manner. To demonstrate ZnO-np toxicity mechanism related to ROS, we detected abnormal autophagic vacuoles accumulation and mitochondria dysfunction after ZnO-np treatment. Furthermore mitochondria membrane potential and adenosine-5′-triphosphate (ATP) production are decreased for culture with ZnO-np. We conclude that ZnO-np leads to cell death through autophagic vacuole accumulation and mitochondria damage in normal skin cells via ROS induction. Accordingly, ZnO-np may cause toxicity and the results highlight and need for careful regulation of ZnO-np production and use.

Keywords : Zinc oxide nanoparticles; Cytotoxicity; Autophagy; Reactive oxygen species (ROS); Mitochondria damage

논문정보

형식Research article
게재일2013년 06월 (BRIC 등록일 2020-02-13)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질
피인용횟수120회 이상 인용된 논문

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