임내균 (한양대학교) | 상위피인용논문 > 한빛사

상위피인용논문

조회962

임내균Nae Gyune Rim

한양대학교

Colloid Surf. B-Biointerfaces, Volume 91, 1 March 2012, Pages 189-197 | https://doi.org/10.1016/j.colsurfb.2011.10.057 Mussel-inspired surface modification of poly(l-lactide) electrospun fibers for modulation of osteogenic differentiation of human mesenchymal stem cells

Nae Gyune Rim^a, Seok Joo Kim^a, Young Min Shin^a, Indong Jun^a, Dong Woo Lim^b, Jung Hwan Park^c, Heungsoo Shin^a,d,e,*

^aDepartment of Bioengineering, College of Engineering, Hanyang University, Seoul, Republic of Korea
^bDepartment of BioNano Engineering, College of Engineering Sciences, Hanyang University, Ansan, Republic of Korea
^c College of BioNano Technology and Gachon BioNano Research Institute, Kyungwon University, Seongnam-si, Republic of Korea
^dInstitute for Bioengineering and Biopharmaceutical Research, Hanyang University, Seoul, Republic of Korea
^e Institute of Aging Society, Hanyang University, Seoul, Republic of Korea

^*Corresponding author : Heungsoo Shin

Abstract

Development of biomaterials to control the fate of stem cells is important for stem cell based regeneration of bone tissue. The objective of this study is to develop functionalized electrospun fibers using a mussel-inspired surface coating to regulate adhesion, proliferation and differentiation of human mesenchymal stem cells (hMSCs). We prepared poly(l-lactide) (PLLA) fibers coated with polydopamine (PD-PLLA). The morphology, chemical composition, and surface properties of fiber were characterized by SEM, AFM, XPS, Raman spectra and water contact angle measurements. Incubation of fibers in dopamine solution for 1 h resulted in formation of polydopamine with only negligible effects on the roughness and hydrophobicity of the fibers. However, PD-PLLA fibers modulated hMSC responses in several aspects. Firstly, adhesion and proliferation of hMSCs cultured on PD-PLLA were significantly enhanced relative to those on PLLA. In addition, the ALP activity of hMSCs cultured on PD-PLLA (1.74 ± 0.14 nmole/DNA/30 min) was significantly higher than on PLLA (0.97 ± 0.07 nmole/DNA/30 min). hMSCs cultured on PD-PLLA showed up-regulation of genes associated with osteogenic differentiation as well as angiogenesis. Furthermore, the calcium deposition from hMSCs cultured on PD-PLLA (41.60 ± 1.74 μg) was significantly greater than that on PLLA (30.15 ± 1.21 μg), which was double-confirmed by alizarin red S staining. Our results suggest that the bio-inspired coating synthetic degradable polymer can be used as a simple technique to render the surface of synthetic biodegradable fibers to be active for directing the specific responses of hMSCs.

Keywords : Poly(l-lactide); Stem cell; Bone tissue engineering; Electrospinning; Polydopamine; Surface modification

논문정보

형식Research article
게재일2012년 03월 (BRIC 등록일 2019-12-17)
연구진국내 연구진
분야 생명과학 > 생물공학
피인용횟수120회 이상 인용된 논문

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