상위피인용논문
- 조회864
한국야쿠르트
Do-Young Park1,†,¤a, Young-Tae Ahn1,†., Se-Hoon Park1, Chul-Sung Huh1,*,¶, Sae-Rom Yoo3, Rina Yu2,4, Mi-Kyung Sung2,5, Robin A. McGregor2,¤b, Myung-Sook Choi2,3,*,¶
1 Korea Yakult Co., Ltd., Yongin, Gyeonggi, Republic of Korea, 2 Center for Food and Nutritional Genomics, Kyungpook National University, Daegu, Republic of Korea, 3 Department of Food Science and Nutrition, Kyungpook National University, Buk-gu, Daegu, Republic of Korea, 4 Department of Food Science and Nutrition, University of Ulsan, Ulsan, Republic of Korea, 5 Department of Food and Nutrition, Sookmyung Women’s University, Seoul, Republic of Korea
*Corresponding author
¤a Current address: School of Biological Science, Seoul National University, Seoul, Republic of Korea
¤b Current address: Institute for Innovation in Biotechnology, School of Biological Sciences, The University of Auckland, Auckland, New Zealand
†These authors contributed equally to this work.
¶These authors also contributed equally to this work.
Abstract
Objective
To investigate the functional effects of probiotic treatment on the gut microbiota, as well as liver and adipose gene expression in diet-induced obese mice.
Design
Male C57BL/6J mice were fed a high-fat diet (HFD) for 8 weeks to induce obesity, and then randomized to receive HFD+probiotic (Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032, n = 9) or HFD+placebo (n = 9) for another 10 weeks. Normal diet (ND) fed mice (n = 9) served as non-obese controls.
Results
Diet-induced obese mice treated with probiotics showed reduced body weight gain and fat accumulation as well as lowered plasma insulin, leptin, total-cholesterol and liver toxicity biomarkers. A total of 151,061 pyrosequencing reads for fecal microbiota were analyzed with a mean of 6,564, 5,274 and 4,464 reads for the ND, HFD+placebo and HFD+probiotic groups, respectively. Gut microbiota species were shared among the experimental groups despite the different diets and treatments. The diversity of the gut microbiota and its composition were significantly altered in the diet-induced obese mice and after probiotic treatment. We observed concurrent transcriptional changes in adipose tissue and the liver. In adipose tissue, pro-inflammatory genes (TNFα, IL6, IL1β and MCP1) were down-regulated in mice receiving probiotic treatment. In the liver, fatty acid oxidation-related genes (PGC1α, CPT1, CPT2 and ACOX1) were up-regulated in mice receiving probiotic treatment.
Conclusions
The gut microbiota of diet-induced obese mice appears to be modulated in mice receiving probiotic treatment. Probiotic treatment might reduce diet-induced obesity and modulate genes associated with metabolism and inflammation in the liver and adipose tissue.
논문정보
- Research article
- 2013년 03월 (BRIC 등록일 2019-04-18)
- 국내 연구진
- 생명과학 > 식품학
- 120회 이상 인용된 논문
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