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J. Am. Chem. Soc., Publication Date (Web): March 30, 2011 | DOI: 10.1021/ja200444t A Mitochondrial-Targeted Two-Photon Probe for Zinc Ion

Abstract

Goutam Masanta^†, Chang Su Lim^§, Hyung Joong Kim^‡, Ji Hee Han^§, Hwan Myung Kim^*†‡, and Bong Rae Cho^*§^†Molecular Science and Technology Research Center, ^‡Division of Energy Systems Research, Ajou University, Suwon 443-749, Korea
^§Department of Chemistry, Korea University, 1-Anamdong, Seoul 136-701, Korea

^*Corresponding Author

Abstract
We report a two-photon probe (SZn-Mito) for mitochondrial zinc ions ([Zn²⁺]_m). This probe shows a 7-fold enhancement of two-photon-excited fluorescence in response to Zn²⁺ with a dissociation constant (K_d^TP) of 3.1 ± 0.1 nM and pH insensitivity in the biologically relevant range, allowing the detection of [Zn²⁺]_m in a rat hippocampal slice at a depth of 100?200 μm without interference from other metal ions through the use of two-photon microscopy.