상위피인용논문
- 조회798
한양대학교
Abstract
Sung In Jeonga, So Yeon Kimb, Seong Kwan Choa, Moo Sang Chonga, Kyung Soo Kimc, Hyuck Kimd, Sang Bong Leea, 1, Young Moo Leea,*
a School of Chemical Engineering, College of Engineering, Hanyang University, Seoul 133-791, Republic of Korea
b Nanomaterials Application Division, Korea Institute of Ceramic, Engineering and Technology, Seoul 153-801, Republic of Korea
c Department of Internal Medicine, College of Medicine, Hanyang University, Seoul 133-791, Republic of Korea
d Department of Thoracic Surgery, College of Medicine, Hanyang University, Seoul 133-791, Republic of Korea
1 Present address: Korea Institute of Industrial Technology, Chungnam 330-825, Republic of Korea.
*Corresponding author : Young Moo Lee
Abstract
Novel tubular scaffolds of marine source collagen and PLGA fibers were fabricated by freeze drying and electrospinning processes for vascular grafts. The hybrid scaffolds, composed of a porous collagen matrix and a fibrous PLGA layer, had an average pore size of 150±50 μm. The electrospun fibrous PLGA layer on the surface of a porous tubular collagen scaffold improved the mechanical strength of the collagen scaffolds in both the dry and wet states. Smooth muscle cells (SMCs)- and endothelial cells (ECs)-cultured collagen/PLGA scaffolds exhibited mechanical properties similar to collagen/PLGA scaffolds unseeded with cells, even after culturing for 23 days. The effect of a mechanical stimulation on the proliferation and phenotype of SMCs and ECs, cultured on collagen/PLGA scaffolds, was evaluated. The pulsatile perfusion system enhanced the SMCs and ECs proliferation. In addition, a significant cell alignment in a direction radial to the distending direction was observed in tissues exposed to radial distention, which is similar to the phenomenon of native vessel tissues in vivo. On the other hand, cells in tissues engineered in the static condition were randomly aligned. Immunochemical analyses showed that the expressions of SM α-actin, SM myosin heavy chain, EC von Willebrand factor, and EC nitric oxide were upregulated in tissues engineered under a mechano-active condition, compared to vessel tissues engineered in the static condition. These results indicated that the co-culturing of SMCs and ECs, using collagen/PLGA hybrid scaffolds under a pulsatile perfusion system, leads to the enhancement of vascular EC development, as well as the retention of the differentiated cell phenotype.
Keywords : Vascular grafts; Bioreactor; Collagen; Scaffold
논문정보
- Research article
- 2007년 02월 (BRIC 등록일 2016-12-01)
- 국내 연구진
- 120회 이상 인용된 논문
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