박제균 (KAIST) | 상위피인용논문 > 한빛사

상위피인용논문

조회743

KAIST

CV File 192 kb

Biomed. Microdevices, February 2007, Volume 9, Issue 1, pp 25-34 | 10.1007/s10544-006-9016-4 A microfluidic platform for 3-dimensional cell culture and cell-based assays

Abstract

Minseok S. Kim¹ , Ju Hun Yeon¹ , Je-Kyun Park^1,*

¹ Department of BioSystems, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea

^*Corresponding author

Abstract
This paper reports a novel microfluidic platform introducing peptide hydrogel to make biocompatible microenvironment as well as realizing in situ cell-based assays. Collagen composite, OPLA and Puramatrix scaffolds are compared to select good environment for human hepatocellular carcinoma cells (HepG2) by albumin measurement. The selected biocompatible self-assembling peptide hydrogel, Puramatrix, is hydrodynamically focused in the middle of main channel of a microfluidic device, and at the same time the cells are 3-dimensionally immobilized and encapsulated without any additional surface treatment. HepG2 cells have been 3-dimensionally cultured in a poly(dimethylsiloxane) (PDMS) microfluidic device for 4 days. The cells cultured in micro peptide scaffold are compared with those cultured by conventional petri dish in morphology and the rate of albumin secretion. By injection of different reagents into either side of the peptide scaffold, the microfluidic device also forms a linear concentration gradient profile across the peptide scaffold due to molecular diffusion. Based on this characteristic, toxicity tests are performed by Triton X-100. As the higher toxicant concentration gradient forms, the wider dead zone of cells in the peptide scaffold represents. This microfluidic platform facilitates in vivo-like 3-dimensional microenvironment, and have a potential for the applications of reliable cell-based screening and assays including cytotoxicity test, real-time cell viability monitoring, and continuous dose-response assay.

Keywords : Microfluidics, 3-D cell culture, Peptide scaffold, Cell-based assays, Cytotoxicity

논문정보

형식Research article
게재일2007년 02월 (BRIC 등록일 2016-06-10)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질
피인용횟수120회 이상 인용된 논문

댓글 작성 로그인

CV 열람하기

연락처 보기