정성인 (한양대학교) | 상위피인용논문 > 한빛사

상위피인용논문

조회837

정성인Sung In Jeong

한양대학교

Biomaterials, Volume 26, Issue 12, April 2005, Pages 1405-1411 | doi:10.1016/j.biomaterials.2004.04.036 Mechano-active tissue engineering of vascular smooth muscle using pulsatile perfusion bioreactors and elastic PLCL scaffolds

Abstract

Sung In Jeong^a, Jae Hyun Kwon^b, Jin Ik Lim^{b, c}, Seung-Woo Cho^{a, d}, Youngmee Jung^b, Won Jun Sung^b, Soo Hyun Kim^b,*, Young Ha Kim^b, Young Moo Lee^a, Byung-Soo Kim^a, Cha Yong Choi^d, Soo-Ja Kim^c

^a School of Chemical Engineering, Hanyang University, Seoul 133-791, Republic of Korea

^b Biomaterials Research Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul 130-650, Republic of Korea

^c Department of Chemistry, Kyunghee University, Seoul 130-701, Republic of Korea

^d School of Chemical Engineering, Seoul National University, Seoul 151-742, Republic of Korea

^*Corresponding author

Abstract

Blood vessels are subjected in vivo to mechanical forces in a form of radial distention, encompassing cyclic mechanical strain due to the pulsatile nature of blood flow. Vascular smooth muscle (VSM) tissues engineered in vitro with a conventional tissue engineering technique may not be functional, because vascular smooth muscle cells (VSMCs) cultured in vitro typically revert from a contractile phenotype to a synthetic phenotype. In this study, we hypothesized that pulsatile strain and shear stress stimulate VSM tissue development and induce VSMCs to retain the differentiated phenotype in VSM engineering in vitro. To test the hypothesis, rabbit aortic smooth muscle cells (SMCs) were seeded onto rubber-like elastic, three-dimensional PLCL [poly(lactide-co-caprolactone), 50:50] scaffolds and subjected to pulsatile strain and shear stress by culturing them in pulsatile perfusion bioreactors for up to 8 weeks. As control experiments, VSMCs were cultured on PLCL scaffolds statically. The pulsatile strain and shear stress enhanced the VSMCs proliferation and collagen production. In addition, a significant cell alignment in a direction radial to the distending direction was observed in VSM tissues exposed to radial distention, which is similar to that of native VSM tissues in vivo, whereas VSMs in VSM tissues engineered in the static condition randomly aligned. Importantly, the expression of SM α-actin, a differentiated phenotype of SMCs, was upregulated by 2.5-fold in VSM tissues engineered under the mechano-active condition, compared to VSM tissues engineered in the static condition. This study demonstrates that tissue engineering of VSM tissues in vitro by using pulsatile perfusion bioreactors and elastic PLCL scaffolds leads to the enhancement of tissue development and the retention of differentiated cell phenotype.

Keywords : Vascular tissue engineering; Pulsatile perfusion bioreactor; Elastic poly-(l-lactide-co-ε-caprolactone) scaffold; Smooth muscle cell

논문정보

형식Research article
게재일2005년 04월 (BRIC 등록일 2016-03-10)
연구진국내 연구진
분야 바이오·의료융합 > 바이오소재
피인용횟수120회 이상 인용된 논문

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