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J. Am. Chem. Soc., Publication Date (Web): June 30, 2011, 133 (29), pp 11132–11135 | DOI: 10.1021/ja205081s Ratiometric Detection of Mitochondrial Thiols with a Two-Photon Fluorescent Probe

Abstract

Chang Su Lim^†, Goutam Masanta^‡, Hyung Joong Kim^§, Ji Hee Han^†, Hwan Myung Kim^*‡§, and Bong Rae Cho ^*†

Department of Chemistry, Korea University, 1-Anamdong, Seoul 136-701, Korea
^‡Molecular Science & Technology Research Center, §Division of Energy Systems Research, Ajou University, Suwon 443-749, Korea

^*Corresponding author : Hwan Myung Kim, Bong Rae Cho

Abstract
We report a ratiometric two-photon probe (SSH-Mito) for mitochondrial thiols. This probe shows a marked blue-to-yellow emission color change in response to RSH, a significant two-photon cross section, good mitochondrial thiol selectivity, low cytotoxicity, and insensitivity to pH over the biologically relevant pH range, allowing the direct visualization of RSH levels in live cells as well as in living tissues at 90-190 μm depth without interference from other biologically relevant species through the use of two-photon microscopy.

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