김우태 (Mayo Clinic, 현 순천향대학교) | 상위피인용논문 > 한빛사

상위피인용논문

조회83

Mayo Clinic, 현 순천향대학교

CV File

Exp. Mol. Med., Oct 2020, 52 (10) | https://doi.org/10.1038/s12276-020-00519-1 DNA end resection and its role in DNA replication and DSB repair choice in mammalian cells

Fei Zhao ^1,#, Wootae Kim ^1,#, Jake A Kloeber^1,2,3, Zhenkun Lou ^1,*

¹Department of Oncology, Mayo Clinic, Rochester, MN 55905 USA
²Mayo Clinic Medical Scientist Training Program, Mayo Clinic, Rochester, MN 55905 USA
³Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, MN 55905 USA

^#These authors contributed equally

^*Corresponding author: correspondence to Zhenkun Lou

Abstract

DNA end resection has a key role in double-strand break repair and DNA replication. Defective DNA end resection can cause malfunctions in DNA repair and replication, leading to greater genomic instability. DNA end resection is initiated by MRN-CtIP generating short, 3'-single-stranded DNA (ssDNA). This newly generated ssDNA is further elongated by multiple nucleases and DNA helicases, such as EXO1, DNA2, and BLM. Effective DNA end resection is essential for error-free homologous recombination DNA repair, the degradation of incorrectly replicated DNA and double-strand break repair choice. Because of its importance in DNA repair, DNA end resection is strictly regulated. Numerous mechanisms have been reported to regulate the initiation, extension, and termination of DNA end resection. Here, we review the general process of DNA end resection and its role in DNA replication and repair pathway choice.

논문정보