한빛사 인터뷰
- 등록2023-10-30
- 조회432
1. Can you please briefly summarize the paper?
We developed 3D liver organoids for modeling tissue function and diseases. It highlights a newly established protocol converting primary human hepatocytes from healthy donor livers into bipotent progenitor cells (hCdHs), and their extension into generating liver organoids for diverse biomedical applications.
The method involves culturing primary human hepatocytes in a specific reprogramming medium to obtain hCdHs, which were then used to create liver organoids for comparison with other liver cell sources. These organoids were extensively analyzed through various assays, molecular profiling, and clinical relevance assessments.
Key findings indicate that hCdH-derived organoids (hCdHOs) exhibited stable stem cell characteristics, surpassed hLOs (human liver organoids) in certain aspects such as EpCAM expression, organoid generation efficiency, and hepatic differentiation. Furthermore, in experiments simulating alcohol-related liver disease (ARLD) and drug-toxicity tests, hCdHOs outperformed hLOs, showcasing stronger ARLD phenotypes and comparable or increased sensitivity responses to drug-induced injuries, making them a valuable platform for regenerative medicine, toxicology testing, and modeling liver diseases.
In conclusion, hCdH-derived liver organoids offer a novel, patient-specific stem cell-based approach with significant potential for various applications in regenerative medicine, toxicology assessments, and disease modeling.
2. Can you please tell us the main difficulties you had in the laboratory work and how you overcame them?
The main difficulty was establishing the model of the organoids, since they are very sensitive structures and almost any stimulus can damage them. This could be resolved by studying more about the topic, asking for help from collaborators and being persistent.
3. Please introduce your laboratory, university or organization to bio-researchers in Korea.
We are the Research Institute of Regenerative Medicine and Stem Cells in Hanyang University. In our investigation group, we are focus in several lines such as:
-The study for applying hepatic cells to humans after differentiating the induced pluripotent stem cells (iPSCs) and the mesenchymal stem cells (MSC) to hepatocyes.
-The study for differentiating fibroblasts from hepatocytes without stem cell stage through direct conversion technique.
-The study for differentiating the corrected cells to hepatocytes through differentiation and direct conversion technique after correcting patient fibroblasts with CRISPR/Cas9.
-The verification of whether hepatocytes induced by direct conversion with induced pluripotent stem cells carry out actual hepatic functions after printing the hepatic cells using the bio 3D technique.
-The study for applying cell therapy as a final goal using a patient-specific artificial organ transplantation or induced hepatocytes directly to humans based on such techniques and studies.
4. Please tell us your experiences and your thoughts related to research activities abroad.
I consider that they are very helpful and it is a great opportunity to enrich our scientific panorama, I highly recommend them. Whoever has the opportunity should do it.
5. Can you provide some advice for younger scientists who have plans to study abroad?
Try it. Don't be afraid, you learn a lot and there are always people willing to help you.
6. Future plan?
At the moment do a new postdoc to gain more experience and maybe in the future apply for a researcher position.
7. Do you have anything else that you would like to tell Korean scientists and students?
You can do it!
You just need perseverance, discipline, and giving yourself time to enjoy your life.
#Liver Organoid
# Human chemically derived hepatic progenitor
# Regenerative medicine
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