이 제품은 GSH-Px elisa kit :: Porcine Glutathione peroxidase ELISA Kit 입니다. 

Principle of the Assay: GPX ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GPX antibody and an GPX-HRP conjugate. The assay sample and buffer are incubated together with GPX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPX concentration since GPX from samples and GPX-HRP conjugate compete for the anti-GPX antibody binding site. Since the number of sites is limited, as more sites are occupied by GPX from the sample, fewer sites are left to bind GPX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPX concentration in each sample is interpolated from this standard curve.

Intended Uses: This GPX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine GPX. This ELISA kit for research use only, not for therapeutic or test applications!

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