Sample Preparation Rapid and efficient preparation of DNA, proteins, and chromatin is vital in performing downstream analyses. Our suite of sample preparation kits are suitable for nearly any workflow, whether it be isolating chromatin for ChIP-Seq or ChIP-PCR applications or preparing DNA from a variety of starting materials for Bisulfite-Seq or MS-PCR. Our sample preparation kits enable you to go directly from extraction to reaction, offering countless possibilities and flexibility in your experimental workflow. Our DNA, protein, and chromatin preparation kits are the starting point for endless research opportunities and downstream applications.
No matter what assay you may be carrying out, proper sample preparation is the initial step that can dictate the success of an entire experiment. EpiGentek's sample preparation products place particular emphasis on the following features: (a) rapid isolation of high quality, ready-to-use DNA, protein, or chromatin samples; (b) efficient yield from trace samples; (c) high flexibility in starting materials; (d) compatibility with various downstream assays or analysis techniques; and (e) specific optimization for use with EpiGentek's assay products.
Popular Kits |
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| | 1. DNA Extraction | Proper DNA preparation is a critical precursor for any assay utilizing isolated DNA as input. Poorly prepared samples can lead to low yield, insufficient purity, and contamination, ultimately compromising the accuracy and reliability of the results. In this article, we will discuss the importance of high-quality DNA for downstream applications, such as PCR, sequencing, and cloning, and the factors that can affect the outcome of an experiment. - Why High-Quality DNA is NecessaryHigh-quality DNA is necessary for most downstream applications, such as PCR, sequencing, and cloning. Poor quality DNA can result in failed experiments, inaccurate results, and even false positives. It is important to note that the purity and concentration of DNA samples can also affect the outcome of an experiment. DNA samples that are contaminated with RNA, proteins, or other contaminants can interfere with the accuracy and reliability of the results. - The Importance of DNA IntegrityThe integrity of the DNA can also impact the success of an experiment. Damaged or degraded DNA may not be suitable for certain applications. For example, if the DNA is damaged or degraded, it may not amplify or sequence accurately, leading to incorrect or incomplete results. - Ensuring High-Quality DNA SamplesTo ensure that DNA samples are of the highest quality possible, it is important to follow established protocols and use reliable reagents and equipment. At EpiGentek we provide a wide range of products for DNA preparation, including [specific products]. Our protocols and methods are based on established best practices and have been validated for performance and reproducibility.
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Cat #P-1017 | |
Cat #P-1007 | |
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Format | spin column | spin column | spin column | spin column | spin column | spin column | Binding Capacity | 5 µg | 5 µg | 5 µg | 5 µg | 5 µg | 5 µg | % Recovery | >70% | >70% | >70% | >70% | >70% | >70% | Starting Material | tissue section | plasma, serum or body fluids | urine | blood leukocytes or cultured mammalian cells | low melting point agarose gel | formalin-fixed, paraffin-embedded tissue | Input Amount | as low as 1 ng | as low as 0.1 ng | 1 ng to 2 µg | 1 ng to 4 µg | 1 ng to 1 µg | 1 ng to 2 µg | Time | 2 hours | 12 minutes | 20 minutes | 20 minutes | lt;15 minutes | 2 hours |
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| P-1004-1 | 50 samples | | P-1004-2 | 100 samples |
| P-1018-050 | 50 samples | | P-1018-100 | 100 samples |
| P-1003-1 | 50 samples | | P-1003-2 | 100 samples |
TuMinute PCR Clean-Up Kit
| Input Type: | DNA | | Research Area: | DNA Methylation | | Target Application: | Sample Isolation | | Vessel Format: | Columns/Tubes |
| P-1005-1 | 50 samples | | P-1005-2 | 100 samples |
DNA Concentrator Kit
Citations (1) | (1)| Input Type: | DNA | | Research Area: | DNA Damage & Repair, DNA Methylation, Next Gen Sequencing | | Target Application: | Sample Isolation | | Vessel Format: | Columns/Tubes |
| P-1006-1 | 50 samples | | P-1006-2 | 100 samples |
| P-1009-1 | 50 samples | | P-1009-2 | 100 samples |
| P-1012-1 | 48 assays | | P-1012-2 | 96 assays |
| P-1017-050 | 50 samples | | P-1017-100 | 100 samples |
| P-1020-48 | 48 assays | | P-1020-96 | 96 assays |
EpiNext DNA Purification HT System
For magnetic bead separation of DNA in DNA library prep, size selection, and cleanup applications
Citations (5)| Input Type: | DNA | | Research Area: | Next Gen Sequencing | | Target Application: | Library Construction | | Vessel Format: | Columns/Tubes |
| P-1063-04 | 4 ml | | P-1063-08 | 8 ml | | P-1063-64 | 64 ml | | P-1063-X4 | 480 ml |
| P-1065-25 | 25 isolations | | P-1065-50 | 50 isolations |
| P-1068-050 | 50 preparations | | P-1068-100 | 100 preparations |
| | 2. RNA Extraction | Purified RNA is used as starting material in a variety of routine applications, such as RT-PCR, cDNA synthesis, Northern blotting, differential display, primer extension, and mRNA selection. Careful preparation of RNA is essential in order to obtain high-quality samples that are suitable for these applications, and can help to ensure reproducible and reliable experimental results. - Importance of High-Quality RNAThe quality of the RNA sample can have a significant impact on the sensitivity and specificity of the assay. Contaminants such as DNA, unwanted RNA species, proteins, and other substances can interfere with the reaction, leading to false negatives or false positives. RNA is also less stable and more prone to hydrolytic degradation when compared with DNA, which can affect the accuracy of the results. Thus, dependable methods for preparing high-quality RNA are of paramount importance. - Applications of RNA preparation kitsOur RNA preparation kits are suitable for the following routine applications: - · RT-PCR
- · cDNA synthesis
- · Northern blotting
- · Differential display
- · Primer extension
- · mRNA selection
- Compatible Sample TypesOur RNA preparation kits are suitable for the quick and efficient isolation of total RNA directly from the following sample types: - · Cultured cells
- · Tissue
- · Cell-free liquid specimens (specifically, saliva and nasopharyngeal swabs)
EpiMag Viral RNA Isolation Kit
| Input Type: | Nasal Swab, Nasopharyngeal Swab, Saliva | | Research Area: | Epidemiology | | Target Application: | Sample Isolation | | Vessel Format: | Beads/Tubes |
| | 3. Chromatin Extraction | - Techniques and Tips for Accurate ResultsChromatin is the complex of DNA and proteins that packages DNA into a smaller volume to fit in the cell, helps to prevent DNA damage, and controls gene expression and DNA replication. Isolation of chromatin samples allows researchers to accurately study protein-DNA interactions within the context of the genome. - Why Proper Techniques are ImportantImproperly prepared chromatin may disrupt or alter the protein-DNA interactions that are being studied, leading to inaccurate or misleading results. If the chromatin isolation process is not carried out carefully, the chromatin may become contaminated with other cellular components, such as proteins and RNA, which can interfere with the protein-DNA interactions of interest. In addition, this process can alter the structure of the chromatin itself, which can also impact the protein-DNA interactions under investigation. Therefore, it is essential to use proper techniques and controls when isolating chromatin in order to ensure that the results of the assay accurately reflect the protein-DNA interactions within the genome. - Recommended Techniques for Chromatin isolationThere are several methods for isolating chromatin, including formaldehyde crosslinking, sonication and micrococcal nuclease digestion, each of these methods have their own advantages and disadvantages. | P-2001-100 | 100 extractions |
| P-2023-100 | 100 preparations |
| | 4. Histone Extraction | Histone extraction is a critical step in epigenetics research, particularly in the study of histone post-translational modifications (PTMs). Histones are small, positively charged proteins around which DNA is wrapped in the cell nucleus. They play a fundamental role in gene regulation and chromatin structure. For accurate quantification and analysis of histone PTMs, researchers need to isolate histone proteins from cellular or tissue samples, an essential process for separating histones from other cellular components such as DNA, RNA, and non-histone proteins. High-quality histone extracts are required for various downstream assays, among which include ELISA-based histone PTM quantification, mass spectrometry analysis, and western blotting. These assays provide insights into the epigenetic regulation of gene expression, which is pivotal in understanding various biological processes like development, disease, and cellular differentiation. EpigenTek’s total histone extraction kits offer a simple, selective, and rapid means of efficiently extracting histone proteins while preserving their modifications, including acetylation, methylation, phosphorylation, ubiquitination, and sumoylation. | | OP-0006-100 | 100 extractions |
| OP-0007-192 | 192 extractions |
| | 5. Nuclear Extraction | Protein preparation and purification are crucial in producing top-notch starting materials for characterizing the structure, function, and interactions of target proteins, both nuclear and whole cell proteins. Downstream studies such as enzymatic and protein quantitation analyses can greatly benefit from proper protein preparation and purification protocols. - Importance of Protein Preparation in Enzymatic AnalysisEnzymatic analysis is especially dependent on proper protein preparation. Variables such as pH, temperature, and the presence of other molecules can greatly impact enzyme activity. Inadequate protein preparation can result in inactive or malformed proteins, which can compromise assay results. Impurities in the protein sample can also negatively affect results by interfering with the assay. By ensuring protein purity and optimal conformation for the assay, proper protein preparation can greatly increase result accuracy and reliability. - Our Protein Preparation Products and ServicesOur company, EpigenTek, offers an extensive range of protein preparation products and services that are designed to provide researchers with high-quality, purified proteins for their downstream applications. From protein isolation and purification to protein characterization and functional assays, our experienced scientists can guide you through every step of the protein preparation process. EpiQuik Nuclear Extraction Kit
For the isolation and extraction of nuclear proteins from cells or tissues while keeping enzymatic activity intact
Citations (476) | (1)| Input Type: | Unisolated Samples | | Research Area: | DNA Methylation, Histone Acetylation, Histone Methylation, Sumoylation | | Target Application: | Sample Isolation | | Vessel Format: | Columns/Tubes |
| OP-0022-100 | 100 extractions |
| | 6. Whole Cell Extraction | EpiQuik Whole Cell Extraction Kit
Citations (30)| Input Type: | Unisolated Samples | | Research Area: | Chromatin & Transcription, DNA Damage & Repair, DNA Methylation, Gene Expression & Silencing, Phosphorylation | | Target Application: | Sample Isolation | | Vessel Format: | Columns/Tubes |
| OP-0003-100 | 100 extractions |
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