[전공: 항체 공학]
석사 과정동안 단일도메인항체(sdAb) 기반 치료용 항체 발굴 및 엔지니어링 연구 수행하였습니다.

[수행 가능 업무]
- 단백질 발현 및 정제 (Protein expression & purification)
   : Recombinant DNA Cloning
   : Protein expression in bacteria (E.coli) system
   : Transient expression in mammalian (Expi293F’) system
   : Affinity chromatography (Protein A, Ni-NTA)
   : Size-exclusion chromatography (SEC) using FPLC

- 항체 발굴 & 엔지니어링 (Antibody screening & engineering)
   : Phage display-based sdAbs library construction
   : Biopanning (solid, bead)
   : Antibody screening (Periplasmic extract-ELISA)
   : CDR grafting & humanization
   : Directed evolution (errorprone PCR, liability motif removal)
   : Rational design using Alphafold3

- 단백질 분석 기술 (Protein analysis)
   : SDS-PAGE, Western blot, ELISA
   : Surface plasmon resonance (SPR)

- 세포 기반 분석 (Cell-based Assay)
   : Flow cytometry (FACS)