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조회 4372  인쇄하기 주소복사 트위터 공유 페이스북 공유 
mRNA Capping Enzyme Activity Is Coupled to an Early Transcription Elongation
Hye-Jin Kim,1 Seok-Ho Jeong,1 Jeong-Hwa Heo,1 Su-Jin Jeong,1 Seong-Tae Kim,2 Hong-Duk Youn,3 Jeong-Whan Han,1 Hyang-Woo Lee,1 and Eun-Jung Cho1*

Department of Biochemistry and Molecular Biology, College of Pharmacy, Sungkyunkwan University, Suwon, Kyonggi-do 440-746,1 School of Medicine, Sungkyunkwan University, Suwon,2 Department of Biochemistry and Molecular Biology, Cancer Research Institute, Seoul National University College of Medicine, Seoul, South Korea3

Received 9 February 2004/ Accepted 19 April 2004

One of the temperature-sensitive alleles of CEG1, a guanylyltransferase subunit of the Saccharomyces cerevisiae capping enzyme, showed 6-azauracil (6AU) sensitivity at the permissive growth temperature, which is a phenotype that is correlated with a transcription elongation defect. This temperature-sensitive allele, ceg1-63, has an impaired ability to induce PUR5 in response to 6AU treatment and diminished enzyme-GMP formation activity. However, this cellular and molecular defect is not primarily due to the preferential degradation of the transcript attributed to a lack of cap structure. Our data suggest that the guanylyltransferase subunit of the capping enzyme plays a role in transcription elongation as well as cap formation. First, in addition to the 6AU sensitivity, ceg1-63 is synthetically lethal with elongation-defective mutations in RNA polymerase II. Secondly, it produces a prolonged steady-state level of GAL1 mRNA after glucose shutoff. Third, it decreases the transcription read through a tandem array of promoter-proximal pause sites in an orientation-dependent manner. Taken together, we present direct evidence that suggests a role of capping enzyme in an early transcription. Capping enzyme ensures the early transcription checkpoint by capping of the nascent transcript in time and allowing it to extend further.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, College of Pharmacy, Sungkyunkwan University, Suwon, Kyonggi-do 440-746, South Korea. Phone: 82-31-290-7781. Fax: 82-31-290-5403.
논문정보
- 형식: Research article
- 게재일: 2004년 07월 (BRIC 등록일 2004-07-02)
- 연구진: 국내연구진태극기
- 분야: Molecular_Biology, Cell_Biology
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