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강희민
강희민 (Heemin Kang) 저자 이메일 보기
The Chinese University of Hong Kong, 현 고려대학교 신소재공학부
 
조회 301  인쇄하기 주소복사 트위터 공유 페이스북 공유 
An In Situ Reversible Heterodimeric Nanoswitch Controlled by Metal‐Ion–Ligand Coordination Regulates the Mechanosensing and Differentiation of Stem Cells
열기 Authors and Affiliations

Abstract

In situ and cytocompatible nanoswitching by external stimuli is highly appealing for reversibly regulating cellular adhesion and functions in vivo. Here, a heterodimeric nanoswitch is designed to facilitate in situ switchable and combinatorial presentation of integrin‐binding cell‐adhesive moieties, such as Mg2+ and Arg‐Gly‐Asp (RGD) ligand in nanostructures. In situ reversible nanoswitching is controlled by convertible coordination between bioactive Mg2+ and bisphosphonate (BP) ligand. A BP‐coated gold‐nanoparticle monomer (BP‐AuNP) on a substrate is prepared to allow in situ assembly of cell‐adhesive Mg2+‐active Mg‐BP nanoparticles (NPs) on a BP‐AuNP surface via Mg2+‐BP coordination, yielding heterodimeric nanostructures (switching “ON”). Ethylenediaminetetraacetic acid (EDTA)‐based Mg2+ chelation allows in situ disassembly of Mg2+‐BP NP, reverting to Mg2+‐free monomer (switching “OFF”). This in situ reversible nanoswitching on and off of cell‐adhesive Mg2+ presentation allows reversible cell adhesion and release in vivo, respectively, and spatiotemporally controls cyclic cell adhesion. In situ heterodimeric assembly of dual RGD ligand‐ and Mg2+‐active RGD‐BP‐Mg2+ NP (switching “Dual ON”) further tunes and promotes focal adhesion, spreading, and differentiation of stem cells. The modular nature of this in situ nanoswitch can accommodate various bioactive nanostructures via metal‐ion–ligand coordination to regulate diverse cellular functions in vivo in reversible and compatible manner.

Keywords : in situ nanoswitches, in vivo cell adhesion, in vivo cell release, metal‐ion–ligand coordination, reversible heterodimers

논문정보
- 형식: Research article
- 게재일: 2018년 09월 (BRIC 등록일 2019-04-08)
- 연구진: 국외연구진
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김성규 (Northwestern University,...)
정희준 (Northwestern University,...)
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프리미엄 Bio일정 Bio일정 프리미엄 안내
제2회 오가노이드 심포지엄 및 핸즈온워크샵 [2019 CHA Organoid Center Symposium]
제2회 오가노이드 심포지엄 및 핸즈온워크샵 [2019 CHA Organoid Center Symposium]
날짜: 2019.05.09
장소: 판교 차바이오컴플렉스 국제회의실
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