1. 논문관련 분야의 소개, 동향, 전망을 설명, 연구과정에서 생긴 에피소드
troduction: Being sessile organisms, plants continuously exposed to a multitude of pathogens, but they usually resist infection using their unique defense systems and compounds, including secondary metabolites. The secondary metabolites inhibits pathogens growth on the plants. However, many of these secondary metabolites are toxic to plants also. To avoid self-toxicity and spatial and temporal delivery of these secondary metabolites transporters play a major role. Several full-size ABCG transporters are involved in the transport of secondary metabolites and pathogen defense in Arabidopsis thaliana and other plant species, which provoked us to identify additional ABCG transporter in Arabidopsis thaliana. To identify additional ABCG transporter, we screened Arabidopsis thaliana mutants of full-size ABCG transporters for hypersensitivity to sclareol, an antifungal compound. We found that atabcg34 mutants were hypersensitive to sclareol and the necrotrophic fungi Alternaria brassicicola.
Perspective: Alternaria brassicicola infection causes dark spots on the leaves of most Brassica species, reducing the yield of economically important oilseed crops. In response to Alternaria brassicicola infection, Arabidopsis thaliana and other Brassicaceae produce and secrete camalexin that imparts resistance to Alternaria brassicicola. Since camalexin is toxic to the plant itself, specific transporters are needed for secretion. In this work, we have shown that the ABC transporter ABCG34 mediates the secretion of camalexin from the epidermal cells to the surface of leaves, and thereby confers resistance to A. brassicicola infection. This work establishes a complete picture of a plant defense system, consisting of a toxic secondary metabolite, its transporter, and the disease phenotype caused by an economically important pathogen.
Trend of this field: Plant pathogens cause huge yield losses. A. brassicicola infection results in dark leaf spots on most Brassica species, including economically-important oilseed crops. Infection by A. brassicicola considerably reduces the quality and quantity of harvested Brassica crops; resulting in annual yield losses of 15 to 70%. To improve the resistance and avoid the yield loss due to pathogen infection, it is necessary to identify key factors, such as ABCG34, which are required for resistance against pathogens. The important factors in economically important Brassica species, such as B. napus and B. oleracea are also possible to identify based on the orthologue search.
2. 연구를 진행했던 소속기관 또는 연구소에 대해 소개 부탁 드립니다.
I entered in Plant Cell Biology Laboratory located in Department of Life Sciences in POSTECH. My principal investigator, Prof. Youngsook Lee, has been supportive throughout my research work. Finally, together we were able to make an interesting and nice story.
3. 연구활동 하시면서 평소 느끼신 점 또는 자부심, 보람
There are always ups and downs during research. Sometimes I felt, I lost my way to proceed further in the project. However, continuous thinking about how to solve the problem, literature study and hard work finally paid off. Now, I feel more confident about-facing the new challenges in the field.
4. 이 분야로 진학하려는 후배들 또는 유학준비생들에게 도움이 되는 말씀을 해 주신다면?
If you like to accept everyday as a challenge and face it with enthusiasm, you are most welcome in the research field. Going abroad for graduation is fascinating as you will not only learn science, but also learn the new culture and language. Being a graduate student, I learnt to never give up, believe in yourself and try your level best to proceed.
5. 연구활동과 관련된 앞으로의 계획이 있으시다면?
I would like to pursue research in future. Now as a post-doctoral fellow and later as faculty. Professor Youngsook Lee is an inspiration for me.