Min-Goo Seoa,b, In-Ohk Ouha, Haeseung Leeb, Paul John L. Geraldinoc, Man Hee Rheeb, OhDeog Kwonb, Dongmi Kwakb,*
aAnimal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon, Gyeongbuk 39660, South Korea
bCollege of Veterinary Medicine, Kyungpook National University, 80 Daehakro, Bukgu, Daegu 41566, South Korea
cDepartment of Biology, School of Arts and Sciences, University of San Carlos, Talamban Campus, Cebu 6000, Philippines
Bovine anaplasmosis is a tick-borne, infectious, non-contagious disease caused by Anaplasma marginale, A. centrale, A. bovis, and zoonotic A. phagocytophilum. Recently, Anaplasma capra detected in goats was identified as a novel zoonotic pathogen. To determine whether A. capra can infect bovines, we used PCR to differentially diagnose Anaplasma spp. in 1,219 South Korean cattle by performing multilocus gene typing and restriction fragment length polymorphism (RFLP). Nucleotide sequencing and phylogenetic analysis detected the 16S rRNA gene of A. bovis and four genes from A. capra in 12 (1.0%) and five (0.4%) cattle, respectively. Supplementary discrimination between A. bovis and A. capra was accomplished by RFLP. The 16S rRNA, msp4, groEL, and gltA genes of A. capra identified in this study had much lower degrees of identity to those in A. centrale and other Anaplasma spp. A. phagocytophilum was not detected in any of the tested cattle. Although the prevalence was low, this study suggests the potential of cattle to serve as reservoirs of A. capra. Thus, further studies are needed to clarify the pathogenesis of A. capra in cattle and its possible involvement in transmission to humans.
Keywords : Anaplasma capra; Anaplasma spp.; Cattle; Genotyping; Multilocus gene typing; Phylogeny