[DEBUG-WINDOW 처리영역 보기]
즐겨찾기  |  뉴스레터  |  오늘의 정보 회원가입   로그인
BRIC홈 농수식품
제품 평가단 모집
스폰서배너광고 안내  배너1 배너2 배너3
전체보기 수산성과 식품성과 인터뷰
사진없음
Irshad Ahmed Hajam (Irshad Ahmed Hajam)
전북대학교 수의과대학
조회 273  인쇄하기 주소복사 트위터 공유 페이스북 공유 
Oral immunization with a novel attenuated Salmonella Gallinarum encoding infectious bronchitis virus spike protein induces protective immune responses against fowl typhoid and infectious bronchitis in chickens
Irshad Ahmed Hajam, Jehyoung Kim and John Hwa Lee*

College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Republic of Korea

*Correspondence: John Hwa Lee

Abstract
Fowl typhoid (FT), a septicemic disease caused by Salmonella Gallinarum (SG), and infectious bronchitis (IB) are two economically important avian diseases that affect poultry industry worldwide. Herein, we exploited a live attenuated SG mutant, JOL967, to deliver spike (S) protein 1 of IB virus (V) to elicit protective immunity against both FT and IB in chickens. The codon optimized S1 nucleotide sequence was cloned in-frame into a prokaryotic constitutive expression vector, pJHL65. Subsequently, empty pJHL65 or recombinant pJHL65-S1 plasmid was electroporated into JOL967 and the resultant clones were designated as JOL2068 and JOL2077, respectively. Our results demonstrated that the chickens vaccinated once orally with JOL2077 elicited significantly (p<0.05) higher IBV-specific humoral and cell-mediated immunity compared to JOL2068 and PBS control groups. Consequently, on challenge with the virulent IBV strain at 28th day post-vaccination, JOL2077 vaccinated birds displayed significantly (p<0.05) lower inflammatory lesions in virus-targeted tissues compared to control groups. Furthermore, 33.3% (2 of 6) of birds vaccinated with JOL2077 vaccine had shown virus recovery from tracheal tissues compared to 100% (6 of 6) recovery obtained in both the control groups. Against wild-type SG lethal challenge, both JOL2077 and JOL2068 vaccinated groups exhibited only 10% mortality compared to 80% mortality observed in PBS control group. In conclusion, we show that JOL2077 can induce efficient IBV- and carrier-specific protective immunity and can act as a bivalent vaccine against FT and IB. Further studies are warranted to investigate the potential of JOL2077 vaccine in broiler and young layer birds.
논문정보
- 형식: Research article
- 게재일: 2018년 09월 (BRIC 등록일 2018-09-19)
- 연구진: 국내연구진 태극기
- 분야: Veterinary_Science
  댓글 0
등록
 
목록
에스엘에스바이오
외부관련링크
Google scholar (by Irshad Ahmed Hajam)
Pubmed (by Irshad Ahmed Hajam)
프리미엄 Bio일정 Bio일정 프리미엄 안내
[BRIC Webinar]플렉서블 유기 인공 신경-이태우 (서울대학교)
[BRIC Webinar]플렉서블 유기 인공 신경-이태우 (서울대학교)
사전접수: ~2019.01.31
날짜: 2019.02.01
Online 개최
[자이스 코리아] 무료 샘플 의뢰 서비스 - Bio Imaging Service 접수 시작
[자이스 코리아] 무료 샘플 의뢰 서비스 - Bio Imaging Service 접수 시작
날짜: 2019.01.21~12.31
장소: 자이스 코리아 서울 데모 센터
[BRIC Webinar]프로바이오틱스 유래 젖산과 장줄기세포의 분화 발달에 관한 연구-이용수(울산대학교 의과대학)
바이오 QC 실무과정 6기 모집
바이오 QC 실무과정 6기 모집
날짜: 2019.03.04~12.09
장소: 성남시 분당구 황새울로 329번길 5
2019 서경배과학재단 신진과학자 연구지원 프로그램 공고
위로가기
농수식품 홈  |  농수식품 문의 및 제안
 |  BRIC소개  |  이용안내  |  이용약관  |  개인정보처리방침  |  이메일무단수집거부
Copyright © BRIC. All rights reserved.  |  문의 member@ibric.org
트위터 트위터    페이스북 페이스북    RSS서비스 RSS