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전체보기 수산성과 식품성과 인터뷰
Irshad Ahmed Hajam (Irshad Ahmed Hajam)
전북대학교 수의과대학
조회 148  인쇄하기 주소복사 트위터 공유 페이스북 공유 
Oral immunization with a novel attenuated Salmonella Gallinarum encoding infectious bronchitis virus spike protein induces protective immune responses against fowl typhoid and infectious bronchitis in chickens
Irshad Ahmed Hajam, Jehyoung Kim and John Hwa Lee*

College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Republic of Korea

*Correspondence: John Hwa Lee

Fowl typhoid (FT), a septicemic disease caused by Salmonella Gallinarum (SG), and infectious bronchitis (IB) are two economically important avian diseases that affect poultry industry worldwide. Herein, we exploited a live attenuated SG mutant, JOL967, to deliver spike (S) protein 1 of IB virus (V) to elicit protective immunity against both FT and IB in chickens. The codon optimized S1 nucleotide sequence was cloned in-frame into a prokaryotic constitutive expression vector, pJHL65. Subsequently, empty pJHL65 or recombinant pJHL65-S1 plasmid was electroporated into JOL967 and the resultant clones were designated as JOL2068 and JOL2077, respectively. Our results demonstrated that the chickens vaccinated once orally with JOL2077 elicited significantly (p<0.05) higher IBV-specific humoral and cell-mediated immunity compared to JOL2068 and PBS control groups. Consequently, on challenge with the virulent IBV strain at 28th day post-vaccination, JOL2077 vaccinated birds displayed significantly (p<0.05) lower inflammatory lesions in virus-targeted tissues compared to control groups. Furthermore, 33.3% (2 of 6) of birds vaccinated with JOL2077 vaccine had shown virus recovery from tracheal tissues compared to 100% (6 of 6) recovery obtained in both the control groups. Against wild-type SG lethal challenge, both JOL2077 and JOL2068 vaccinated groups exhibited only 10% mortality compared to 80% mortality observed in PBS control group. In conclusion, we show that JOL2077 can induce efficient IBV- and carrier-specific protective immunity and can act as a bivalent vaccine against FT and IB. Further studies are warranted to investigate the potential of JOL2077 vaccine in broiler and young layer birds.
- 형식: Research article
- 게재일: 2018년 09월 (BRIC 등록일 2018-09-19)
- 연구진: 국내연구진 태극기
- 분야: Veterinary_Science
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Google scholar (by Irshad Ahmed Hajam)
Pubmed (by Irshad Ahmed Hajam)
프리미엄 Bio일정 Bio일정 프리미엄 안내
2018 Ajou Biomedical International Symposium / Genoproteomic Signature of Human Diseases
2018 Ajou Biomedical International Symposium / Genoproteomic Signature of Human Diseases
날짜: 2018.11.26
장소: 아주대학교의료원 별관 대강당 (수원)
Human Genome Meeting 2019
Human Genome Meeting 2019
초록접수: ~2018.12.31
사전접수: ~2018.12.31
날짜: 2019.04.24~26
장소: 이화여자대학교 삼성홀
International Forum on Medical Innovation of Cell & Bio Therapy
International Forum on Medical Innovation of Cell & Bio Therapy
사전접수: ~2018.11.21
날짜: 2018.11.23
장소: 서울대학교병원 의생명연구원 1층 강당(대학로 소재)
[K-Genome Symposium 2018] 유전체, 헬스빅데이터, 그리고 정밀의료
[K-Genome Symposium 2018] 유전체, 헬스빅데이터, 그리고 정밀의료
날짜: 2018.11.19
장소: 아주대학교의료원 별관 대강당
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