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전체보기 농림성과 수산성과 인터뷰
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김미라 (Mi-Ra Kim)
식품의약품안전평가원
조회 162  인쇄하기 주소복사 트위터 공유 페이스북 공유 
A rapid real-time PCR method to differentiate between mottled skate (Beringraja pulchra) and other skate and ray species
Mi-Ra Kim1, Kisung Kwon1, Yoo-Kyung Jung, Tae Sun Kang*

New Hazardous Substance Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, 187Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do 28159, South Korea

1
These authors contributed equally to this work.

*Corresponding author :Tae Sun Kang

Abstract
Skates and rays are commercially important fish in South Korea, and among them, Beringraja pulchra has the highest economic value. However, the similar morphological traits among skates and rays are often exploited for seafood fraud. Here, we designed both Beringraja pulchra-specific and skate-universal primer sets, capable of detecting short sequences in the cytochrome oxidase subunit I gene, and developed highly sensitive and reliable quantitative real-time PCR (qPCR) assays to differentiate between Beringraja pulchra and other skate and ray species. AΔCq method based on differences in the amplification efficiency was developed, validated, and then used to confirm the presence of Beringraja pulchra in twenty-six commercial skate products. The averageΔCq value obtained for other skate species (18.94±3.46) was significantly higher than that of Beringraja pulchra (1.18±0.15). For on-site applications, we developed an ultra-fast qPCR assay, allowing for completion of the entire analytical procedure within 30 min.

Keywords : Genetic identification, Seafood authentication, Ultra-fast qPCR, Cytochrome oxidase subunit I, Amplification effciency
논문정보
- 형식: Research article
- 게재일: 2018년 07월 (BRIC 등록일 2018-08-09)
- 연구진: 국내연구진 태극기
- 분야: Food_Technology
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