Hyunjong Yua,1, Moon-Won Leeb,1, Hyukjin Shina, Kyung-Min Parkc,*, Pahn-Shick Changa,d,e,*
aDepartment of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea
bDepartment of Public Health Sciences, Graduate School, Korea University, Seoul 02841, Republic of Korea
cDepartment of Food Science and Biotechnology, Wonkwang University, Iksan 54538, Republic of Korea
dCenter for Food and Bioconvergence, Seoul National University, Seoul 08826, Republic of Korea
eResearch Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea
1Equal contribution as first authors.
Erythorbyl laurate is a potential food additive as a multi-functional emulsifier having antioxidant and antimicrobial activities. In this study, a gas-solid-liquid multiphase system (GSL-MPS) was established to enhance the production yield of erythorbyl laurate in a lipase-catalyzed solvent-free synthesis. The significant reaction variables were optimized as follows: substrate molar ratio of 2:1 (lauric acid:erythorbic acid) and enzyme concentration of 120 mg/mL (840 PLU/mL). Under these conditions, the maximum production yield in GSL-MPS was 13.974 mg/mL, which is 8.60- and 4.26-fold higher than the yields obtained in an organic solvent monophase system (OS-MPS) and a solid-liquid biphase system (SL-BPS), respectively. Moreover, the operational stability of the immobilized lipase was significantly improved in GSL-MPS compared with OS-MPS. These results indicate that GSL-MPS can be an enzymatic reaction system facilitating efficient production of ester compounds as a means of increasing production yields and the reusability of the immobilized lipase.
Keywords : Erythorbyl laurate, Lipase-catalyzed solvent-free synthesis, Gas-solid-liquid multiphase system, Production yield, Immobilized lipase