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대메뉴안내: 농수식품
농림성과 수산성과 식품성과 인터뷰
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Nitin Machindra Kamble(Nitin Machindra Kamble)
전북대학교 수의과대학
조회92  주소복사  소셜네트워크로 공유하기
Intracellular delivery of HA1 subunit antigen through attenuated Salmonella Gallinarum act as a bivalent vaccine against fowl typhoid and low pathogenic H5N3 virus

Nitin Machindra Kamble, Kim Je Hyoung and John Hwa Lee*

College of Veterinary Medicine, Chonbuk National University, Iksan Campus, Jeonju 570-752, Republic of Korea

*Correspondence : John Hwa Lee

Abstract
Introduction of novel inactivated oil-emulsion vaccines against different strains of prevailing and emerging low pathogenic avian influenza (LPAI) viruses is not an economically viable option for poultry. Engineering attenuated Salmonella Gallinarum (S. Gallinarum) vaccine delivering H5 LPAI antigens can be employed as a bivalent vaccine against fowl typhoid and LPAI viruses, while still offering economic viability and sero-surveillance capacity. In this study, we developed a JOL1814 bivalent vaccine candidate against LPAI virus infection and fowl typhoid by engineering the attenuated S. Gallinarum to deliver the globular head (HA1) domain of hemagglutinin protein from H5 LPAI virus through pMMP65 constitutive expression plasmid. The important feature of the developed JOL1814 was the delivery of the HA1 antigen to cytosol of peritoneal macrophages. Immunization of chickens with JOL1814 produced significant level of humoral, mucosal, cellular and IL-2, IL-4, IL-17 and IFN-γ cytokine immune response against H5 HA1 and S. Gallinarum antigens in the immunized chickens. Post-challenge, only the JOL1814 immunized chicken showed significantly faster clearance of H5N3 virus in oropharyngeal and cloacal swabs, and 90% survival rate against lethal challenge with a wild type S. Gallinarum. Furthermore, the JOL1814 immunized were differentiated from the H5N3 LPAI virus infected chickens by matrix (M2) gene-specific real-time PCR. In conclusion, the data from the present showed that the JOL1814 can be an effective bivalent vaccine candidate against H5N3 LPAI and fowl typhoid infection in poultry while still offering sero-surveillance property against H5 avian influenza virus.

논문정보
- 형식: Research article
- 게재일: 2017년 08월 (BRIC 등록일 2017-08-14)
- 연구진: 국내연구진 태극기
- 분야: Veterinary_Science
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Google scholar (by Nitin Machindra Kamble)
Pubmed (by Nitin Machindra Kamble)
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[GE APAC Fast Trak] 다운스트림 바이오프로세스 개발과정 (DEV2)
[GE APAC Fast Trak] 다운스트림 바이오프로세스 개발과정 (DEV2)
사전접수: ~2017.10.13
날짜: 2017.10.16~20
장소: 인천시 연수구 송도미래로 9 BRC 2동 2층 GE Healthcare APAC Fast Trak Center
혁신적 진단 플랫폼 기술 개발 공모전
혁신적 진단 플랫폼 기술 개발 공모전
날짜: 2017.10.11
주관: (주)제우스
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