Dong-wan Koh, Jae-woong Park, Jung-hoon Lim, Myeong-Jai Yea, Dae-young Bang*
Lotte R & D Center, 19, Yangpyeong-ro 19-gil, Yeongdeungpo-gu, Seoul 07209, Republic of Korea
*Corresponding author : Dae-young Bang
A novel, rapid, simultaneous analysis method for five sugars (fructose, glucose, sucrose, maltose, and lactose) and eight sugar alcohols (erythritol, xylitol, sorbitol, mannitol, inositol, maltitol, lactitol, and isomalt) was developed using UPLC-ELSD, without derivatization. The analysis conditions, including the gradient conditions, modifier concentration and column length, were optimized. Thirteen sugars and sugar alcohols were separated well and the resolution of their peaks was above 1.0. Their optimum analysis condition can be analyzed within 15 min. Standard curves for sugars and sugar alcohols with concentrations of 5.0-0.1% and 2.0?0.05% are presented herein, and their correlation coefficients are found to be above 0.999 and the limit of detection (LOD) was around 0.006-0.018%. This novel analysis system can be used for foodstuffs such as candy, chewing gum, jelly, chocolate, processed chocolate products, and snacks containing 0.21-46.41% of sugars and sugar alcohols.
Keywords : Sugar, Sugar alcohol, UPLC, ELSD, Foodstuffs