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조회189  주소복사  소셜네트워크로 공유하기
A Lactococcus lactis BFE920 feed vaccine expressing a fusion protein composed of the OmpA and FlgD antigens from Edwardsiella tarda was significantly better at protecting olive flounder (Paralichthys olivaceus) from edwardsiellosis than single antigen vaccines

Bo Ram Becka, Soon Ho Leeb, Daniel Kimb, Ji Hye Parka, Hyun Kyung Leeb, San-Sung Kwonb, Kwan Hee Leea, Jae Il Leec, Seong Kyu Songa, b, *

Research Center, Immunus Co., Ltd., Business Incubation Center 205, Handong University, Pohang 37554, Republic of Korea
bSchool of Life Science, Handong University, Pohang 37554, Republic of Korea
c GyeongSangbuk-Do Fisheries Technology Center, Yeongdeok 36426, Republic of Korea

* Corresponding author.

Edwardsiellosis is a major fish disease that causes a significant economic damage in the aquaculture industry. Here, we assessed vaccine efficacy after feeding oral vaccines to olive flounder (Paralichthys olivaceus), either L. lactis BFE920 expressing Edwardsiella tarda outer membrane protein A (OmpA), flagellar hook protein D (FlgD), or a fusion antigen of the two. Feed vaccination was done twice with a one-week interval. Fish were fed regular feed adsorbed with the vaccines. Feed vaccination was given over the course of one week to maximize the interaction between the feed vaccines and the fish intestine. Flounder fed the vaccine containing the fusion antigen had significantly elevated levels T cell genes (CD4-1, CD4-2, and CD8α), type 1 helper T cell (Th1) subset indicator genes (T-bet and IFN-γ), and antigen-specific antibodies compared to the groups fed the single antigen-expressing vaccines. Furthermore, the superiority of the fusion vaccine was also observed in survival rates when fish were challenged with E. tarda: OmpA-FlgD-expressing vaccine (82.5% survival); FlgD-vaccine (55.0%); OmpA-vaccine (50%); WT L. lactis BFE920 (37.5%); Ctrl (10%). In addition, vaccine-fed fish exhibited increased weight gain (∼20%) and a decreased feed conversion ratio (∼20%) during the four week vaccination period. Flounder fed the FlgD-expressing vaccine, either the single or the fusion form, had significantly increased expression of TLR5M, IL-1β, and IL-12p40, suggesting that the FlgD may be a ligand of olive flounder TLR5M receptor or closely related to the TLR5M pathway. In conclusion, the present study demonstrated that olive flounder fed L. lactis BFE920 expressing a fusion antigen composed of E. tarda OmpA and FlgD showed a strong protective effect against edwardsiellosis indicating this may be developed as an E. tarda feed vaccine.

Keywords : Lactococcus lactis BFE920, Edwardsiella tarda, Edwardsiellosis, Fusion antigen, Feed vaccine, Oral vaccine, Olive flounder

- 형식: Research article
- 게재일: 2017년 09월 (BRIC 등록일 2017-08-11)
- 연구진: 국내연구진 태극기
- 분야: Immunology, Marine_Biology, Veterinary_Science
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Google scholar (by Bo Ram Beck)
Pubmed (by Bo Ram Beck)
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[GE APAC Fast Trak] 다운스트림 바이오프로세스 개발과정 (DEV2)
[GE APAC Fast Trak] 다운스트림 바이오프로세스 개발과정 (DEV2)
사전접수: ~2017.10.13
날짜: 2017.10.16~20
장소: 인천시 연수구 송도미래로 9 BRC 2동 2층 GE Healthcare APAC Fast Trak Center
혁신적 진단 플랫폼 기술 개발 공모전
혁신적 진단 플랫폼 기술 개발 공모전
날짜: 2017.10.11
주관: (주)제우스
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