[DEBUG-WINDOW 처리영역 보기]
BRIC을 시작페이지로 회원가입    로그인
배너1 배너2 스폰서배너광고 안내
오늘의 BRIC정보
트위터 페이스북
검색 뉴스레터 안내
좋은 연구문화 만들기
Bio일정 프리미엄(유료) 등록이란?
바이오 형광사진
실험의 달인들
Biojob 프리미엄(유료) 등록이란?
대메뉴안내: 농수식품
농림성과 수산성과 식품성과 인터뷰
송성규 (Seong Kyu Song) 저자 이메일 보기
조회 362  인쇄하기 주소복사 트위터 공유 페이스북 공유 
A Lactococcus lactis BFE920 feed vaccine expressing a fusion protein composed of the OmpA and FlgD antigens from Edwardsiella tarda was significantly better at protecting olive flounder (Paralichthys olivaceus) from edwardsiellosis than single antigen vaccines

Bo Ram Becka, Soon Ho Leeb, Daniel Kimb, Ji Hye Parka, Hyun Kyung Leeb, San-Sung Kwonb, Kwan Hee Leea, Jae Il Leec, Seong Kyu Songa, b, *

Research Center, Immunus Co., Ltd., Business Incubation Center 205, Handong University, Pohang 37554, Republic of Korea
bSchool of Life Science, Handong University, Pohang 37554, Republic of Korea
c GyeongSangbuk-Do Fisheries Technology Center, Yeongdeok 36426, Republic of Korea

* Corresponding author.

Edwardsiellosis is a major fish disease that causes a significant economic damage in the aquaculture industry. Here, we assessed vaccine efficacy after feeding oral vaccines to olive flounder (Paralichthys olivaceus), either L. lactis BFE920 expressing Edwardsiella tarda outer membrane protein A (OmpA), flagellar hook protein D (FlgD), or a fusion antigen of the two. Feed vaccination was done twice with a one-week interval. Fish were fed regular feed adsorbed with the vaccines. Feed vaccination was given over the course of one week to maximize the interaction between the feed vaccines and the fish intestine. Flounder fed the vaccine containing the fusion antigen had significantly elevated levels T cell genes (CD4-1, CD4-2, and CD8α), type 1 helper T cell (Th1) subset indicator genes (T-bet and IFN-γ), and antigen-specific antibodies compared to the groups fed the single antigen-expressing vaccines. Furthermore, the superiority of the fusion vaccine was also observed in survival rates when fish were challenged with E. tarda: OmpA-FlgD-expressing vaccine (82.5% survival); FlgD-vaccine (55.0%); OmpA-vaccine (50%); WT L. lactis BFE920 (37.5%); Ctrl (10%). In addition, vaccine-fed fish exhibited increased weight gain (∼20%) and a decreased feed conversion ratio (∼20%) during the four week vaccination period. Flounder fed the FlgD-expressing vaccine, either the single or the fusion form, had significantly increased expression of TLR5M, IL-1β, and IL-12p40, suggesting that the FlgD may be a ligand of olive flounder TLR5M receptor or closely related to the TLR5M pathway. In conclusion, the present study demonstrated that olive flounder fed L. lactis BFE920 expressing a fusion antigen composed of E. tarda OmpA and FlgD showed a strong protective effect against edwardsiellosis indicating this may be developed as an E. tarda feed vaccine.

Keywords : Lactococcus lactis BFE920, Edwardsiella tarda, Edwardsiellosis, Fusion antigen, Feed vaccine, Oral vaccine, Olive flounder

- 형식: Research article
- 게재일: 2017년 09월 (BRIC 등록일 2017-08-11)
- 연구진: 국내연구진 태극기
- 분야: Immunology, Marine_Biology, Veterinary_Science
  댓글 0
Google scholar (by Seong Kyu Song)
Pubmed (by Seong Kyu Song)
프리미엄 Bio일정 Bio일정 프리미엄 안내
2018년 경북대학교 대학원 의과학과 학술대회•Open Lab
2018년 경북대학교 대학원 의과학과 학술대회•Open Lab
사전접수: ~2018.08.14
날짜: 2018.08.22~23
장소: 경북대학교 글로벌플라자 (22일) 경북대학교 의과대학 (동인동)
2018년 부산대학교 의학전문대학원 의과학과 Open Lab
2018년 부산대학교 의학전문대학원 의과학과 Open Lab
사전접수: ~2018.08.20
날짜: 2018.08.28
장소: 부산대학교 의학전문대학원(양산) 통합행정동 116호
이전페이지로 돌아가기 맨위로 가기

BRIC 홈    BRIC 소개    회원    검색    문의/FAQ    광고    후원
Copyright © BRIC. All rights reserved. Contact member@ibric.org