[DEBUG-WINDOW 처리영역 보기]
BRIC을 시작페이지로 회원가입    로그인
BRIC농수식품
   
통합검색
배너1 배너2 배너3 스폰서배너광고 안내
오늘의 BRIC정보
모바일 BRIC RSS
트위터 페이스북
검색 뉴스레터 안내
좋은 연구문화 만들기
Bio일정
Bio일정
 
Bio일정 프리미엄(유료) 등록이란?
2017년 10월달 Bio일정을 확인하세요.
실험
실험
바이오 형광사진
실험의 달인들
Bio마켓
Bio마켓
BioJob
BioJob
Biojob 프리미엄(유료) 등록이란?
커뮤니티
커뮤니티
전체메뉴
대메뉴안내: 농수식품
농림성과 수산성과 식품성과 인터뷰
장규환
장규환(Guehwan Jang) 저자 이메일 보기
경북대학교
 
조회136  주소복사  소셜네트워크로 공유하기
Prevalence, complete genome sequencing and phylogenetic analysis of porcine deltacoronavirus in South Korea, 2014–2016

G. Jang1, K.-K. Lee2, S.-H. Kim2 and C. Lee1,*

1
Animal Virology Laboratory, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu, Korea
2Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Gimcheon, Korea

* Correspondence : C. Lee, School of Life Sciences, College of Natural Sciences, Kyungpook National University, Daegu, Korea.

Summary
Porcine deltacoronavirus (PDCoV) is a newly emerged enterotropic swine coronavirus that causes enteritis and diarrhoea in piglets. Here, a nested reverse transcription (RT)-PCR approach for the detection of PDCoV was developed to identify and characterize aetiologic agent(s) associated with diarrhoeal diseases in piglets in South Korea. A PCR-based method was applied to investigate the presence of PDCoV in 683 diarrhoeic samples collected from 449 commercial pig farms in South Korea from January 2014 to December 2016. The molecular-based survey indicated a relatively high prevalence of PDCoV (19.03%) in South Korea. Among those, the monoinfection of PDCoV (9.66%) and co-infection of PDCoV (6.30%) with porcine epidemic diarrhoea (PEDV) were predominant in diarrhoeal samples. The full-length genomes or the complete spike genes of the most recent strains identified in 2016 (KNU16-07, KNU16-08 and KNU16-11) were sequenced and analysed to characterize PDCoV currently prevalent in South Korea. We found a single insertion-deletion signature and dozens of genetic changes in the spike (S) genes of the KNU16 isolates. Phylogenetic analysis based on the entire genome and spike protein sequences of these strains indicated that they are most closely related to other Korean isolates grouped with the US strains. However, Korean PDCoV strains formed different branches within the same cluster, implying continuous evolution in the field. Our data will advance the understanding of the molecular epidemiology and evolutionary characteristics of PDCoV circulating in South Korea.

Keywords: full-length genome;nested RT-PCR;PDCoV;PDCoV;prevalence

논문정보
- 형식: Research article
- 게재일: 2017년 07월 (BRIC 등록일 2017-08-04)
- 연구진: 국내연구진 태극기
- 분야: Veterinary_Science
댓글등록
작성자
 
목록
싸토리우스코리아
싸토리우스코리아 스폰서배너광고 안내
외부관련링크
Google scholar (by Guehwan Jang)
Pubmed (by Guehwan Jang)
프리미엄 Bio일정 Bio일정 프리미엄 안내
[GE APAC Fast Trak] 다운스트림 바이오프로세스 개발과정 (DEV2)
[GE APAC Fast Trak] 다운스트림 바이오프로세스 개발과정 (DEV2)
사전접수: ~2017.10.13
날짜: 2017.10.16~20
장소: 인천시 연수구 송도미래로 9 BRC 2동 2층 GE Healthcare APAC Fast Trak Center
혁신적 진단 플랫폼 기술 개발 공모전
혁신적 진단 플랫폼 기술 개발 공모전
날짜: 2017.10.11
주관: (주)제우스
이전페이지로 돌아가기 맨위로 가기
 

BRIC 홈    BRIC 소개    회원    검색    문의/FAQ    광고    후원
Copyright © BRIC. All rights reserved. Contact member@ibric.org

 
에펜도르프(T:1577-4395)