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농림성과 수산성과 식품성과 인터뷰
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조회 507  인쇄하기 주소복사 트위터 공유 페이스북 공유 
Prevalence, complete genome sequencing and phylogenetic analysis of porcine deltacoronavirus in South Korea, 2014–2016

G. Jang1, K.-K. Lee2, S.-H. Kim2 and C. Lee1,*

1
Animal Virology Laboratory, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu, Korea
2Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Gimcheon, Korea

* Correspondence : C. Lee, School of Life Sciences, College of Natural Sciences, Kyungpook National University, Daegu, Korea.

Summary
Porcine deltacoronavirus (PDCoV) is a newly emerged enterotropic swine coronavirus that causes enteritis and diarrhoea in piglets. Here, a nested reverse transcription (RT)-PCR approach for the detection of PDCoV was developed to identify and characterize aetiologic agent(s) associated with diarrhoeal diseases in piglets in South Korea. A PCR-based method was applied to investigate the presence of PDCoV in 683 diarrhoeic samples collected from 449 commercial pig farms in South Korea from January 2014 to December 2016. The molecular-based survey indicated a relatively high prevalence of PDCoV (19.03%) in South Korea. Among those, the monoinfection of PDCoV (9.66%) and co-infection of PDCoV (6.30%) with porcine epidemic diarrhoea (PEDV) were predominant in diarrhoeal samples. The full-length genomes or the complete spike genes of the most recent strains identified in 2016 (KNU16-07, KNU16-08 and KNU16-11) were sequenced and analysed to characterize PDCoV currently prevalent in South Korea. We found a single insertion-deletion signature and dozens of genetic changes in the spike (S) genes of the KNU16 isolates. Phylogenetic analysis based on the entire genome and spike protein sequences of these strains indicated that they are most closely related to other Korean isolates grouped with the US strains. However, Korean PDCoV strains formed different branches within the same cluster, implying continuous evolution in the field. Our data will advance the understanding of the molecular epidemiology and evolutionary characteristics of PDCoV circulating in South Korea.

Keywords: full-length genome;nested RT-PCR;PDCoV;PDCoV;prevalence

논문정보
- 형식: Research article
- 게재일: 2017년 07월 (BRIC 등록일 2017-08-04)
- 연구진: 국내연구진 태극기
- 분야: Veterinary_Science
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2018년 경북대학교 대학원 의과학과 학술대회•Open Lab
2018년 경북대학교 대학원 의과학과 학술대회•Open Lab
사전접수: ~2018.08.14
날짜: 2018.08.22~23
장소: 경북대학교 글로벌플라자 (22일) 경북대학교 의과대학 (동인동)
2018년 부산대학교 의학전문대학원 의과학과 Open Lab
2018년 부산대학교 의학전문대학원 의과학과 Open Lab
사전접수: ~2018.08.20
날짜: 2018.08.28
장소: 부산대학교 의학전문대학원(양산) 통합행정동 116호
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