[DEBUG-WINDOW 처리영역 보기]
BRIC을 시작페이지로 회원가입    로그인
BRIC농수식품
   
통합검색
배너1 배너2 스폰서배너광고 안내
오늘의 BRIC정보
모바일 BRIC RSS
트위터 페이스북
검색 뉴스레터 안내
좋은 연구문화 만들기
Bio일정
Bio일정
 
Bio일정 프리미엄(유료) 등록이란?
실험
실험
바이오 형광사진
실험의 달인들
Bio마켓
Bio마켓
BioJob
BioJob
Biojob 프리미엄(유료) 등록이란?
커뮤니티
커뮤니티
전체메뉴
대메뉴안내: 농수식품
농림성과 수산성과 식품성과 인터뷰
사진없음
G.I. Godahewa(G.I. Godahewa)
제주대학교
조회43  주소복사  소셜네트워크로 공유하기
Manganese-superoxide dismutase (MnSOD), a role player in seahorse (Hippocampus abdominalis) antioxidant defense system and adaptive immune system

N.C.N. Pereraa, 1, G.I. Godahewaa, 1, Seongdo Leea, Myoung-Jin Kima, Jee Youn Hwangb, Mun Gyeong Kwonb, Seong Don Hwangb, Jehee Leea, *

a
 Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province 63243, Republic of Korea
b Aquatic Disease Control Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea

1
These authors contributed equally to this work.

* Corresponding author

Abstract
Manganese superoxide dismutase (MnSOD) is a metaloenzyme that catalyzes dismutation of the hazardous superoxide radicals into less hazardous H2O2 and H2O. Here, we identified a homolog of MnSOD from big belly seahorse (Hippocampus abdominalis; HaMnSOD) and characterized its structural and functional features. HaMnSOD transcript possessed an open reading frame (ORF) of 672 bp which codes for a peptide of 223 amino acids. Pairwise alignment showed that HaMnSOD shared highest identity with rock bream MnSOD. Results of the phylogenetic analysis of HaMnSOD revealed a close proximity with rock bream MnSOD which was consistent with the result of homology alignment. The intense expression of HaMnSOD was observed in the ovary, followed by the heart and the brain. Further, immune related responses of HaMnSOD towards pathogenic stimulation were observed through bacterial and viral challenges. Highest HaMnSOD expression in response to stimulants Edwardsiella tarda, Streptococcus iniae, lipopolysaccharide (LPS), and polyinosinic-polycytidylic acid (Poly I:C) was observed in the late stage in the blood tissue. Xanthine/xanthine oxidase assay (XOD assay) indicated the ROS-scavenging ability of purified recombinant HaMnSOD (rHaMnSOD). The optimum conditions for the SOD activity of rHaMnSOD were pH 9 and the 25 °C. Collectively, the results obtained through the expressional analysis profiles and the functional assays provide insights into potential immune related and antioxidant roles of HaMnSOD in the big belly seahorse.

Keywords : Antioxidant, Immune response, MnSOD, XOD assay

논문정보
- 형식: Research article
- 게재일: 2017년 07월 (BRIC 등록일 2017-08-03)
- 연구진: 국내연구진 태극기
- 분야: Immunology, Marine_Biology, Veterinary_Science
댓글등록
작성자
 
목록
마텍무역
마텍무역 스폰서배너광고 안내
외부관련링크
Google scholar (by G.I. Godahewa)
Pubmed (by G.I. Godahewa)
프리미엄 Bio일정 Bio일정 프리미엄 안내
[GE APAC Fast Trak] 다운스트림 바이오프로세스 개발과정 (DEV2)
[GE APAC Fast Trak] 다운스트림 바이오프로세스 개발과정 (DEV2)
사전접수: ~2017.10.13
날짜: 2017.10.16~20
장소: 인천시 연수구 송도미래로 9 BRC 2동 2층 GE Healthcare APAC Fast Trak Center
혁신적 진단 플랫폼 기술 개발 공모전
혁신적 진단 플랫폼 기술 개발 공모전
날짜: 2017.10.11
주관: (주)제우스
이전페이지로 돌아가기 맨위로 가기
 

BRIC 홈    BRIC 소개    회원    검색    문의/FAQ    광고    후원
Copyright © BRIC. All rights reserved. Contact member@ibric.org