[DEBUG-WINDOW 처리영역 보기]
BRIC을 시작페이지로 회원가입    로그인
BRIC농수식품
   
통합검색
배너1 배너2 스폰서배너광고 안내
오늘의 BRIC정보
모바일 BRIC RSS
트위터 페이스북
검색 뉴스레터 안내
좋은 연구문화 만들기
Bio일정
Bio일정
 
Bio일정 프리미엄(유료) 등록이란?
2018 IUBMB SEOUL Plenary Lecture VOD
실험
실험
바이오 형광사진
실험의 달인들
Bio마켓
Bio마켓
BioJob
BioJob
Biojob 프리미엄(유료) 등록이란?
커뮤니티
커뮤니티
전체메뉴
대메뉴안내: 농수식품
농림성과 수산성과 식품성과 인터뷰
A.M. Abd El-Aty
A.M. Abd El-Aty (A.M. Abd El-Aty) 저자 이메일 보기
건국대학교 수의과대학, Cairo University
 
조회 474  인쇄하기 주소복사 트위터 공유 페이스북 공유 
A quick and effective methodology for analyzing dinotefuran and its highly polar metabolites in plum using liquid chromatography-tandem mass spectrometry

Md. Musfiqur Rahmana, A.M. Abd El-Atyb, c,*, Md. Humayun Kabira, Hyung Suk Chunga, Han Sol Leea, Fazil Hacımüftüoğlud, Ji Hoon Jeonge, Byung-Joon Changf, Ho-Chul Shinc, Jae-Han Shima,*

a
Natural Products Chemistry Lab., College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea
b Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211-Giza, Egypt
c Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea
d Department of Soil Sciences and Plant Nutrition, Faculty of Agriculture, Ataturk University, 25240 Erzurum, Turkey
e Department of Pharmacology, College of Medicine, Chung-Ang University, 221, Heuksuk-dong, Dongjak-gu, Seoul 156-756, Republic of Korea
f Department of Veterinary Anatomy, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea

*Corresponding authors at: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea (A. M. Abd El-Aty) and Natural Products Chemistry Lab., College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea (J.-H. Shim).

Abstract
A simple and effective method was developed for analyzing dinotefuran and its three metabolites (MNG, UF, and DN) in plum using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Due to the polarity and high water miscibility, dinotefuran and some of its metabolites (especially DN) have some limitations to be extracted with acetonitrile and salt following the “QuEChERS” sample preparation methodology. Alternatively, the samples were extracted with methanol, and purified with dispersive-solid phase extraction procedure (d-SPE) using primary secondary amine (PSA) and C18 sorbents after filtration, and mass up. Due to the suppression effect originated from plum matrix, matrix-matched calibration curves, which provided good linearity with coefficient of determination (R2) ≥ 0.998, were used for quantification of all analytes. Blank plum samples fortified with 2 spiking levels (10 × LOQ and 50 × LOQ) yielded satisfactory recoveries for all tested analytes in the range of 83.01 to 110.18% with relative standard deviation (RSD) ≤ 8.91. The method was successfully applied to field-incurred plum samples and dinotefuran and all metabolites were positively detected and quantified. In conclusion, we suggest that the method can be expanded to polar compounds having solvent and partitioning problems in any of the versions of QuEChERS.

Keywords : Dinotefuran; Polar metabolites; Matrix effect; Plum; QuEChERS

논문정보
- 형식: Research article
- 게재일: 2017년 07월 (BRIC 등록일 2017-07-25)
- 연구진: 국내(교신)+국외 연구진 태극기
- 분야: Food_Technology
  댓글 0
등록
 
목록
(주)신코
외부관련링크
Google scholar (by A.M. Abd El-Aty)
Pubmed (by A.M. Abd El-Aty)
프리미엄 Bio일정 Bio일정 프리미엄 안내
강북삼성병원 개원 50주년 기념 국제심포지엄
강북삼성병원 개원 50주년 기념 국제심포지엄
사전접수: ~2018.07.18
날짜: 2018.07.20
장소: 중앙일보빌딩 호암아트홀 (서울시 중구 서소문로 100)
의료기기 임상시험의 이해 과정
의료기기 임상시험의 이해 과정
날짜: 2018.06.27~29
장소: 경기도 성남시 분당구 판교역로 182 한국반도체산업협회 건물 9층
[The 12th Catholic International Stem Cell Symposium] 제12차 가톨릭국제줄기세포심포지엄
[The 12th Catholic International Stem Cell Symposium] 제12차 가톨릭국제줄기세포심포지엄
초록접수: ~2018.06.10
사전접수: ~2018.06.24
날짜: 2018.06.29
장소: 가톨릭대학교 성의교정 마리아홀(서울 반포)
이전페이지로 돌아가기 맨위로 가기
 

BRIC 홈    BRIC 소개    회원    검색    문의/FAQ    광고    후원
Copyright © BRIC. All rights reserved. Contact member@ibric.org

 
에펜도르프코리아