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농림성과 수산성과 식품성과 인터뷰
Jayesh Puthumana(Jayesh Puthumana)
조회672  주소복사  소셜네트워크로 공유하기
Nine co-localized cytochrome P450 genes of the CYP2N, CYP2AD, and CYP2P gene families in the mangrove killifish Kryptolebias marmoratus genome: Identification and expression in response to B[α]P, BPA, OP, and NP

Jayesh Puthumanaa, Bo-Mi Kima, b, Chang-Bum Jeonga, Duck-Hyun Kima, Hye-Min Kanga, Jee-Hyun Jungc, Il-Chan Kimd, Un-Ki Hwange, Jae-Seong Leea,*

Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea
b Unit of Polar Genomics, Korea Polar Research Institute, Incheon 21990, South Korea
c Oil and POPs Research Group, Korea Institute of Ocean Science and Technology, Geoje 53201, South Korea
d Division of Polar Life Sciences, Korea Polar Research Institute, Incheon 21990, South Korea
e Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Fisheries Research & Development Institute, Incheon 46083, South Korea

*Corresponding author : Jae-Seong Lee

The CYP2 genes are the largest and most diverse cytochrome P450 (CYP) subfamily in vertebrates. We have identified nine co-localized CYP2 genes (∼55 kb) in a new cluster in the genome of the highly resilient ecotoxicological fish model Kryptolebias marmoratus. Molecular characterization, temporal and tissue-specific expression pattern, and response to xenobiotics of these genes were examined. The CYP2 gene clusters were characterized and designated CYP2N22-23, CYP2AD12, and CYP2P16-20. Gene synteny analysis confirmed that the cluster in K. marmoratus is similar to that found in other teleost fishes, including zebrafish. A gene duplication event with diverged catalytic function was observed in CYP2AD12. Moreover, a high level of divergence in expression was observed among the co-localized genes. Phylogeny of the cluster suggested an orthologous relationship with similar genes in zebrafish and Japanese medaka. Gene expression analysis showed that CYP2P19 and CYP2N20 were consecutively expressed throughout embryonic development, whereas CYP2P18 was expressed in all adult tissues, suggesting that members of each CYP2 gene family have different physiological roles even though they are located in the same cluster. Among endocrine-disrupting chemicals (EDCs), benzo[α]pyrene (B[α]P) induced expression of CYP2N23, bisphenol A (BPA) induced CYP2P18 and CYP2P19, and 4-octylphenol (OP) induced CYP2AD12, but there was no significant response to 4-nonylphenol (NP), implying differential catalytic roles of the enzyme. In this paper, we identify and characterize a CYP2 gene cluster in the mangrove killifish K. marmoratus with differing catalytic roles toward EDCs. Our findings provide insights on the roles of nine co-localized CYP2 genes and their catalytic functions for better understanding of chemical-biological interactions in fish.

Keywords : Cytochrome P450; gene cluster; killifish; Kryptolebias marmoratus; endocrine disrupting chemical

- 형식: Research article
- 게재일: 2017년 03월 (BRIC 등록일 2017-04-03)
- 연구진: 국내연구진 태극기
- 분야: Marine_Biology
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[8월 9일 수요일] 품질분석 시험방법 밸리데이션 (Validation of Analytical Procedures)
[8월 9일 수요일] 품질분석 시험방법 밸리데이션 (Validation of Analytical Procedures)
사전접수: ~2017.08.08
날짜: 2017.08.09
장소: 구로디지털단지 우림이비지센터2차 1406호 (2호선 구로디지털단지역 3번출구 도보 10분)
2017 부산대학교 의학전문대학원 의과학과 Open Lab
2017 부산대학교 의학전문대학원 의과학과 Open Lab
사전접수: ~2017.08.16
날짜: 2017.08.21
장소: 부산대학교 의학전문대학원(양산) 통합행정동 116호
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