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허은미
허은미 (Eun-Mi Hur) 저자 이메일 보기
한국과학기술연구원, UST
 
조회 2363  인쇄하기 주소복사 트위터 공유 페이스북 공유 
Anisotropically organized three-dimensionalculture platform for reconstruction of ahippocampal neural network

So Hyun Kim1,*,†, Sun-Kyoung Im2,3,*, Soo-Jin Oh2,3,*, Sohyeon Jeong1,4, Eui-Sung Yoon1,4, C. Justin Lee2,5,6, Nakwon Choi1,4 & Eun-Mi Hur2,3,5

1
Center for BioMicrosystems, Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea. 2 Center for Neuroscience, Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea. 3 Convergence Research Center for Diagnosis, Treatment and Care System of Dementia, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea. 4 Department of Biomedical Engineering, Korea University of Science and Technology (UST), Daejeon 34113, Korea. 5 Department of Neuroscience, Korea University of Science and Technology (UST), Daejeon 34113, Korea. 6 The KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 02841, Korea.

*
These authors contributed equally to this work.
Present address: SK Biopharmaceuticals Co., Ltd., Seongnam 13494, Korea.

Correspondence to Nakwon Choi or Eun-Mi Hur.

Abstract
In native tissues, cellular and acellular components are anisotropically organized and often aligned in specific directions, providing structural and mechanical properties for actuating biological functions. Thus, engineering alignment not only allows for emulation of native tissue structures but might also enable implementation of specific functionalities. However, achieving desired alignment is challenging, especially in three-dimensional constructs. By exploiting the elastomeric property of polydimethylsiloxane and fibrillogenesis kinetics of collagen, here we introduce a simple yet effective method to assemble and align fibrous structures in a multi-modular three-dimensional conglomerate. Applying this method, we have reconstructed the CA3?CA1 hippocampal neural circuit three-dimensionally in a monolithic gel, in which CA3 neurons extend parallel axons to and synapse with CA1 neurons. Furthermore, we show that alignment of the fibrous scaffold facilitates the establishment of functional connectivity. This method can be applied for reconstructing other neural circuits or tissue units where anisotropic organization in a multi-modular structure is desired.

논문정보
TOP5
2017년 선정
- 형식: Research article
- 게재일: 2017년 02월 (BRIC 등록일 2017-02-02)
- 연구진: 국내연구진태극기
- 분야: Neuroscience, Biotechnology
관련 보도자료
뇌 신경회로망 실험실 배양 틀에 구현 성공
뇌 신경회로망 실험실 배양 틀에 구현 성공    서울경제  |  2017.02.13
신경세포의 지지체 역할을 하는 콜라겐과 신경세포를 3차원 배지 역할을 하는 투명 고무 재질의 배양 틀에 올려놓고 몸속 세포의 정렬 방향과 같게 3차원(3D) 배양하는 데 성공했다.
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관련인물
김경태 (포항공과대학교)
김덕호 (University of Washington)
김소현 (한국과학기술연구원)
양인홍 (Johns Hopkins University)
오수진 (한국과학기술연구원)
임선경 (한국과학기술연구원)
최낙원 (한국과학기술연구원)
관련분야 논문보기
Neuroscience

Biotechnology

외부링크
Google (by Eun-Mi Hur)
Pubmed (by Eun-Mi Hur)
프리미엄 Bio일정 Bio일정 프리미엄 안내
2018 Ajou Biomedical International Symposium / Genoproteomic Signature of Human Diseases
2018 Ajou Biomedical International Symposium / Genoproteomic Signature of Human Diseases
날짜: 2018.11.26
장소: 아주대학교의료원 별관 대강당 (수원)
Human Genome Meeting 2019
Human Genome Meeting 2019
초록접수: ~2018.12.31
사전접수: ~2018.12.31
날짜: 2019.04.24~26
장소: 이화여자대학교 삼성홀
International Forum on Medical Innovation of Cell & Bio Therapy
International Forum on Medical Innovation of Cell & Bio Therapy
사전접수: ~2018.11.21
날짜: 2018.11.23
장소: 서울대학교병원 의생명연구원 1층 강당(대학로 소재)
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