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조윤경 (Yoon-Kyoung Cho) 저자 이메일 보기
조회 830  인쇄하기 주소복사 트위터 공유 페이스북 공유 
Exodisc for Rapid, Size-Selective, and Efficient Isolation and Analysis of Nanoscale Extracellular Vesicles from Biological Samples

Hyun-Kyung Woo†‡, Vijaya Sunkara†‡, Juhee Park, Tae-Hyeong Kim, Ja-Ryoung Han, Chi-Ju Kim†⊥, Hyun-Il Choi§, Yoon-Keun Kim§∥, and Yoon-Kyoung Cho*†⊥ 

Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea
§ Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea
Institute of MD Healthcare, Seoul 03923, Republic of Korea
Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 44919, Republic of Korea

*Corresponding Author

Author Contributions
These authors contributed equally to this work.

Extracellular vesicles (EVs) are cell-derived, nanoscale vesicles that carry nucleic acids and proteins from their cells of origin and show great potential as biomarkers for many diseases, including cancer. Efficient isolation and detection methods are prerequisites for exploiting their use in clinical settings and understanding their physiological functions. Here, we presented a rapid, label-free, and highly sensitive method for EV isolation and quantification using a lab-on-a-disc integrated with two nanofilters (Exodisc). Starting from raw biological samples, such as cell-culture supernatant (CCS) or cancer-patient urine, fully automated enrichment of EVs in the size range of 20-600 nm was achieved within 30 min using a tabletop-sized centrifugal microfluidic system. Quantitative tests using nanoparticle-tracking analysis confirmed that the Exodisc enabled >95% recovery of EVs from CCS. Additionally, analysis of mRNA retrieved from EVs revealed that the Exodisc provided >100-fold higher concentration of mRNA as compared with the gold-standard ultracentrifugation method. Furthermore, on-disc enzyme-linked immunosorbent assay using urinary EVs isolated from bladder cancer patients showed high levels of CD9 and CD81 expression, suggesting that this method may be potentially useful in clinical settings to test urinary EV-based biomarkers for cancer diagnostics.

Keywords: bladder cancer; ELISA; extracellular vesicles; lab-on-a-disc; size-based filtration

2017년 선정
- 형식: Research article
- 게재일: 2017년 01월 (BRIC 등록일 2017-01-19)
- 연구진: 국내연구진태극기
관련 보도자료
소변으로 암 진단한다...소변에서 '나노 소포체' 분리하는 기술 내놓아
소변으로 암을 진단할 수 있는 길이 열렸습니다. 국내 연구팀이 소변이나 혈액에서 암 진단에 필요한 물질만 효과적으로 채집하는 기술을 내놓았습니다. 조직검사에 집중됐던 암의 진단과 치료를 소변으로 간단하게 진단할 수 있습니다.
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조윤경 님 전체논문보기 >
Christopher Ko (삼성바이오에피스)
Vijaya Sunkara (UNIST)
우현경 (UNIST)
이범석 (삼성종합기술원)
이정근 (삼성종합기술원)
Google (by Yoon-Kyoung Cho)
Pubmed (by Yoon-Kyoung Cho)
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2018 Ajou Biomedical International Symposium / Genoproteomic Signature of Human Diseases
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초록접수: ~2018.12.31
사전접수: ~2018.12.31
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