[DEBUG-WINDOW 처리영역 보기]
즐겨찾기  |  뉴스레터  |  오늘의 정보  |  e브릭몰e브릭몰 회원가입   로그인
BRIC홈 한국을 빛내는 사람들
랩박스 - Neuroscience
스폰서배너광고 안내  배너1
전체보기 한빛사논문 추천논문 인터뷰 그이후 한빛사통계
전영준
전영준 (Young-Jun Jeon) 저자 이메일 보기
The Ohio State University
 
조회 4231  인쇄하기 주소복사 트위터 공유 페이스북 공유 
Role of MYC-Regulated Long Noncoding RNAs in Cell Cycle Regulation and Tumorigenesis

Taewan Kim*, Young-Jun Jeon*, Ri Cui, Ji-Hoon Lee, Yong Peng, Sung-Hak Kim, Esmerina Tili, Hansjuerg Alder and Carlo M. Croce

Affiliations of authors: Department of Molecular and Cellular Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX (TK); Department of Molecular Virology, Immunology and Medical Genetics (TK, YJJ, RC, ET, HA, CMC), Department of Neurological Surgery (SHK), and Department of Anesthesiology (ET), Wexner Medical Center, The Ohio State University, OH; School of Biological Sciences, Seoul National University and National Creative Research Initiative Center for Symbiosystem, Seoul, Republic of Korea (JHL); State Key Laboratory of Biotherapy/Collaborative Innovation Center of Biotherapy, West China Hospital, Sichuan University, Chengdu, China (YP); School of Life Sciences and Biotechnology, Korea University, Republic of Korea (SHK).

*Authors contributed equally to this work.
Correspondence to: Carlo M. Croce, MD, 1082 BRT, 460 West 12th Ave, Columbus, OH 43210

Abstract

Background: The functions of long noncoding RNAs (lncRNAs) have been identified in several cancers, but the roles of lncRNAs in colorectal cancer (CRC) are less well understood. The transcription factor MYC is known to regulate lncRNAs and has been implicated in cancer cell proliferation and tumorigenesis.

Methods: CRC cells and tissues were profiled to identify lncRNAs differentially expressed in CRC, from which we further selected MYC-regulated lncRNAs. We used luciferase promoter assay, ChIP, RNA pull-down assay, deletion mapping assay, LC-MS/MS and RNA immunoprecipitation to determine the mechanisms of MYC regulation of lncRNAs. Moreover, soft agar assay and in vivo xenograft experiments (four athymic nude mice per group) provided evidence of MYC-regulated lncRNAs in cancer cell transformation and tumorigenesis. The Kaplan-Meier method was used for survival analyses. All statistical tests were two-sided.

Results: We identified lncRNAs differentially expressed in CRC (P < .05, greater than two-fold) and verified four lncRNAs upregulated and two downregulated in CRC cells and tissues. We further identified MYC-regulated lncRNAs, named MYCLos. The MYC-regulated MYCLos may function in cell proliferation and cell cycle by regulating MYC target genes such as CDKN1A (p21) and CDKN2B (p15), suggesting new regulatory mechanisms of MYC-repressed target genes through lncRNAs. RNA binding proteins including HuR and hnRNPK are involved in the function of MYCLos by interacting with MYCLo-1 and MYCLo-2, respectively. Knockdown experiments also showed that MYCLo-2, differentially expressed not only in CRC but also in prostate cancer, has a role in cancer transformation and tumorigenesis.

Conclusions: Our results provide novel regulatory mechanisms in MYC function through lncRNAs and new potential lncRNA targets of CRC.

논문정보
- 형식: Research article
- 게재일: 2015년 04월 (BRIC 등록일 2018-05-14)
- 연구진: 국내+국외 연구진
- 분야: Cancer Biology/Oncology, Molecular_Biology, Genomics
- 피인용횟수: 최근 3년간 60회 이상 인용된 논문
m6A 변형에 의해 유도되는 RNA의 내부적 붕괴[Mol. Cell]
박옥현
발표: 박옥현 (고려대학교)
일자: 2019년 6월 21일 (금) 오후 02시 (한국시간)
언어: 한국어
참석자 접수신청하기
CRISPR-BetaBinomial(CB2) 기법을 이용한 CRISPR pooled screen data 분석[Genome Res.]
정현환
발표: 정현환 (Baylor College of Medicine, Texas C...)
일자: 2019년 6월 26일 (수) 오전 10시 (한국시간)
언어:
참석자 접수신청하기

  댓글 2
회원작성글 김성학  (2015-02-18 10:57)
전박사 고생 많았어. 앞으로 좋은 일만 가득하기를 바래. 그리고 OSU를 빛낸 것도 자랑스럽고....
회원작성글 Serendipity  (2015-02-20 06:03)
태완이랑 둘이 수고 많았다. 따뜻해지면 내쉬빌로 한번 내려와 소주나 한잔 하게.
등록
 
라이카코리아
관련링크
전영준 님 전체논문보기 >
관련인물
김태완 (The University of Texas M...)
관련분야 논문보기
Cancer Biology/Oncology

Molecular_Biology

Biochemistry

외부링크
Google (by Young-Jun Jeon)
Pubmed (by Young-Jun Jeon)
프리미엄 Bio일정 Bio일정 프리미엄 안내
[BRIC Webinar]CRISPR-BetaBinomial(CB2) 기법을 이용한 CRISPR pooled screen data 분석-정현환 (Baylor College of Medicine, Texas Children’s Hospital)
대구경북과학기술원(DGIST) 뇌·인지과학전공 3-DAY INTENSE BRAIN BOOTCAMP
대구경북과학기술원(DGIST) 뇌·인지과학전공 3-DAY INTENSE BRAIN BOOTCAMP
사전접수: ~2019.06.30
날짜: 2019.07.10~12
장소: 대구경북과학기술원 (DGIST) 뇌·인지과학전공
[BRIC Webinar]m6A변형에 의해 유도되는 RNA의 내부적 붕괴-박옥현(고려대학교)
[재직자 무료교육] 비임상시험 이해(2일과정)
[재직자 무료교육] 비임상시험 이해(2일과정)
사전접수: ~2019.06.24
날짜: 2019.06.26~07.05
장소: 청주시 흥덕구 오송생명1로 194-41 충북산학융합본부
[대졸 미취업자]바이오QC 실무과정 7기 모집
[대졸 미취업자]바이오QC 실무과정 7기 모집
날짜: 2019.09.02~06.12
장소: 성남시 분당구 황새울로 329번길 5
2019 대성해강미생물포럼
2019 대성해강미생물포럼
날짜: 2019.06.20
장소: 웨스틴 조선호텔 서울 오키드룸
위로가기
한빛사 홈  |  한빛사FAQ  |  한빛사 문의 및 제안
 |  BRIC소개  |  이용안내  |  이용약관  |  개인정보처리방침  |  이메일무단수집거부
Copyright © BRIC. All rights reserved.  |  문의 member@ibric.org
트위터 트위터    페이스북 페이스북   유튜브 유튜브    RSS서비스 RSS
에펜도르프코리아