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조회4953  인쇄  주소복사  소셜네트워크로 공유하기
Formate-driven growth coupled with H2 production

Yun Jae Kim1*, Hyun Sook Lee1,2*, Eun Sook Kim1*, Seung Seob Bae1,2*, Jae Kyu Lim1,2, Rie Matsumi3, Alexander V. Lebedinsky4, Tatyana G. Sokolova4, Darya A. Kozhevnikova4, Sun-Shin Cha1,2, Sang-Jin Kim1,2, Kae Kyoung Kwon1,2, Tadayuki Imanaka3, Haruyuki Atomi3, Elizaveta A. Bonch-Osmolovskaya4, Jung-Hyun Lee1,2 & Sung Gyun Kang1,2

1Korea Ocean Research & Development Institute, PO Box 29, Ansan 425-600, Korea. 2Department of Marine Biotechnology, University of Science and Technology, Daejeon 305-333, Korea. 3Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan. 4Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-Letiya Oktyabrya 7/2, 117312, Moscow, Russia.

Although a common reaction in anaerobic environments, the conversion of formate and water to bicarbonate and H2 (with a change in Gibbs free energy of ΔG° = +1.3kJ mol-1) has not been considered energetic enough to support growth of microorganisms. Recently, experimental evidence for growth on formate was reported for syntrophic communities of Moorella sp. strain AMP and a hydrogen-consuming Methanothermobacter species and of Desulfovibrio sp. strain G11 and Methanobrevibacter arboriphilus strain AZ1. The basis of the sustainable growth of the formate-users is explained by H2 consumption by the methanogens, which lowers the H2 partial pressure, thus making the pathway exergonic2. However, it has not been shown that a single strain can grow on formate by catalysing its conversion to bicarbonate and H2. Here we report that several hyperthermophilic archaea belonging to the Thermococcus genus are capable of formate-oxidizing, H2-producing growth. The actual ΔG values for the formate metabolism are calculated to range between -8 and -20 kJ mol -1 under the physiological conditions where Thermococcus onnurineus strain NA1 are grown. Furthermore, we detected ATP synthesis in the presence of formate as a sole energy source. Gene expression profiling and disruption identified the gene cluster encoding formate hydrogen lyase, cation/proton antiporter and formate transporter, which were responsible for the growth of T. onnurineus NA1 on formate. This work shows formate-driven growth by a single microorganism with protons as the electron acceptor, and reports the biochemical basis of this ability.

- 형식: Research article
- 게재일: 2010년 09월 (BRIC 등록일 2010.09.16)
- 연구진: 국내(교신)+국외 연구진 태극기
- 분야: Molecular_Biology, Genetics, Genomics
Complete prevention of blood loss with self-sealing haemostatic needles
발표: 신미경 (KAIST)
일자: 2016년 10월 31일 (월) 오후 03시 (한국시간)
언어: 한국어
참석자 접수신청하기
Obesity and Risk of Secondary Primary Cancer in Cancer Survivors: 암경험자의 이차암 위험 예방 및 장기건강관리
발표: 박상민 (서울대학교 의과대학)
일자: 2016년 11월 15일 (화) 오전 10시 (한국시간)
언어: 한국어
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마텍무역 스폰서배너광고 안내
   외부 관련 링크
Google scholar (by Sung Gyun Kang)
Pubmed (by Sung Gyun Kang)
강성균 님의 Lab/개인 홈페이지
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관련뉴스 2
   BRIC 관련 링크
강성균 님의 한빛사 논문보기
관련분야 한빛사 논문보기
Molecular_Biology  Genetics  Genomics
강성균 님의 관련인물
  김윤재 (한국해양연구원)
  이현숙 (한국해양과학기술원)
  김은숙 (한국해양연구원)
  배승섭 (한국해양연구원)
  이정현 (한국해양과학기술원)
  임재규 (한국해양과학기술원)
프리미엄 Bio일정 Bio일정 프리미엄 안내
[한국바이오협회] QbD Section 심화 과정
[한국바이오협회] QbD Section 심화 과정
날짜: 2016.11.09~11
장소: 한국반도체산업협회 9층 강의장(경기 성남시 분당구 판교역로 182)
Global Trends in Live Cell Kinetic Assays
Global Trends in Live Cell Kinetic Assays
사전접수: ~2016.10.27
날짜: 2016.11.01
장소: 충청북도 C&V센터 (오송)
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