[DEBUG-WINDOW 처리영역 보기]
BRIC을 시작페이지로 회원가입    로그인
BRIC한국을 빛내는 사람들
   
통합검색
배너1 스폰서배너광고 안내
오늘의 BRIC정보
바로가기 메뉴 설정하기
실험Q&A
채용정보
뉴스
모바일 BRIC RSS
트위터 페이스북
검색 뉴스레터 안내
바이오형광사진공모전
오빠, 나 살찐거 같지?
꽃잎처럼 보이는 PAG 컬럼
금빛섬광
전체보기
Bio일정
Bio일정
 
Bio일정 프리미엄(유료) 등록이란?
한빛사 웨비나 - 이종민
Bio마켓
Bio마켓
BioJob
BioJob
Biojob 프리미엄(유료) 등록이란?
전체메뉴
대메뉴안내: 한빛사
한빛사논문 추천논문 상위피인용논문 한빛사인터뷰 한빛사그이후
강성균
강성균(Sung Gyun Kang) 저자 이메일 보기
한국해양연구원
 
조회4237  인쇄  주소복사  소셜네트워크로 공유하기
Formate-driven growth coupled with H2 production

Yun Jae Kim1*, Hyun Sook Lee1,2*, Eun Sook Kim1*, Seung Seob Bae1,2*, Jae Kyu Lim1,2, Rie Matsumi3, Alexander V. Lebedinsky4, Tatyana G. Sokolova4, Darya A. Kozhevnikova4, Sun-Shin Cha1,2, Sang-Jin Kim1,2, Kae Kyoung Kwon1,2, Tadayuki Imanaka3, Haruyuki Atomi3, Elizaveta A. Bonch-Osmolovskaya4, Jung-Hyun Lee1,2 & Sung Gyun Kang1,2

1Korea Ocean Research & Development Institute, PO Box 29, Ansan 425-600, Korea. 2Department of Marine Biotechnology, University of Science and Technology, Daejeon 305-333, Korea. 3Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan. 4Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-Letiya Oktyabrya 7/2, 117312, Moscow, Russia.

Although a common reaction in anaerobic environments, the conversion of formate and water to bicarbonate and H2 (with a change in Gibbs free energy of ΔG° = +1.3kJ mol-1) has not been considered energetic enough to support growth of microorganisms. Recently, experimental evidence for growth on formate was reported for syntrophic communities of Moorella sp. strain AMP and a hydrogen-consuming Methanothermobacter species and of Desulfovibrio sp. strain G11 and Methanobrevibacter arboriphilus strain AZ1. The basis of the sustainable growth of the formate-users is explained by H2 consumption by the methanogens, which lowers the H2 partial pressure, thus making the pathway exergonic2. However, it has not been shown that a single strain can grow on formate by catalysing its conversion to bicarbonate and H2. Here we report that several hyperthermophilic archaea belonging to the Thermococcus genus are capable of formate-oxidizing, H2-producing growth. The actual ΔG values for the formate metabolism are calculated to range between -8 and -20 kJ mol -1 under the physiological conditions where Thermococcus onnurineus strain NA1 are grown. Furthermore, we detected ATP synthesis in the presence of formate as a sole energy source. Gene expression profiling and disruption identified the gene cluster encoding formate hydrogen lyase, cation/proton antiporter and formate transporter, which were responsible for the growth of T. onnurineus NA1 on formate. This work shows formate-driven growth by a single microorganism with protons as the electron acceptor, and reports the biochemical basis of this ability.

논문정보
- 형식: Research article
- 게재일: 2010년 09월 (BRIC 등록일 2010.09.16)
- 연구진: 국내(교신)+국외 연구진 태극기
- 분야: Molecular_Biology, Genetics, Genomics
Identification of Genetic Factors that Modify Clinical Onset of Huntington’s Disease
이종민
발표: 이종민 (Massachusetts General Hospital / Ha...)
일자: 2015년 9월 4일 (금) 오전 11시 (한국시간)
언어: 한국어
대기자 접수신청하기

댓글등록
작성자
 
목록
필코리아테크놀로지
필코리아테크놀로지 스폰서배너광고 안내
   외부 관련 링크
Google scholar (by Sung Gyun Kang)
Pubmed (by Sung Gyun Kang)
강성균 님의 Lab/개인 홈페이지
관련뉴스 1
관련뉴스 2
   BRIC 관련 링크
강성균 님의 한빛사 논문보기
관련분야 한빛사 논문보기
Molecular_Biology  Genetics  Genomics
강성균 님의 관련인물
  김윤재 (한국해양연구원)
  이현숙 (한국해양과학기술원)
  김은숙 (한국해양연구원)
  배승섭 (한국해양연구원)
  이정현 (한국해양과학기술원)
  임재규 (한국해양과학기술원)
프리미엄 Bio일정 Bio일정 프리미엄 안내
2015년도 한국분자세포생물학회 정기학술대회
2015년도 한국분자세포생물학회 정기학술대회
날짜: 2015.09.21~23
장소: 코엑스 1층 Grand Ballroom
이전페이지로 돌아가기 맨위로 가기
 

BRIC 홈    BRIC 소개    회원    검색    문의/FAQ    광고
Copyright © BRIC. All rights reserved. Contact member@ibric.org