[DEBUG-WINDOW 처리영역 보기]
BRIC을 시작페이지로 회원가입    로그인
BRIC한국을 빛내는 사람들
배너1 배너2 배너3 배너4 스폰서배너광고 안내
오늘의 BRIC정보
바로가기 메뉴 설정하기
트위터 페이스북
검색 뉴스레터 안내
Whole brain imaging showing retinal ganglion cell projections in adult mouse
거미줄 (The spider web)
Bio일정 프리미엄(유료) 등록이란?
Biojob 프리미엄(유료) 등록이란?
대메뉴안내: 한빛사
한빛사논문 추천논문 상위피인용논문 한빛사인터뷰 한빛사그이후
강성균(Sung Gyun Kang) 저자 이메일 보기
조회4035  인쇄  주소복사  소셜네트워크로 공유하기
Formate-driven growth coupled with H2 production

Yun Jae Kim1*, Hyun Sook Lee1,2*, Eun Sook Kim1*, Seung Seob Bae1,2*, Jae Kyu Lim1,2, Rie Matsumi3, Alexander V. Lebedinsky4, Tatyana G. Sokolova4, Darya A. Kozhevnikova4, Sun-Shin Cha1,2, Sang-Jin Kim1,2, Kae Kyoung Kwon1,2, Tadayuki Imanaka3, Haruyuki Atomi3, Elizaveta A. Bonch-Osmolovskaya4, Jung-Hyun Lee1,2 & Sung Gyun Kang1,2

1Korea Ocean Research & Development Institute, PO Box 29, Ansan 425-600, Korea. 2Department of Marine Biotechnology, University of Science and Technology, Daejeon 305-333, Korea. 3Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan. 4Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-Letiya Oktyabrya 7/2, 117312, Moscow, Russia.

Although a common reaction in anaerobic environments, the conversion of formate and water to bicarbonate and H2 (with a change in Gibbs free energy of ΔG° = +1.3kJ mol-1) has not been considered energetic enough to support growth of microorganisms. Recently, experimental evidence for growth on formate was reported for syntrophic communities of Moorella sp. strain AMP and a hydrogen-consuming Methanothermobacter species and of Desulfovibrio sp. strain G11 and Methanobrevibacter arboriphilus strain AZ1. The basis of the sustainable growth of the formate-users is explained by H2 consumption by the methanogens, which lowers the H2 partial pressure, thus making the pathway exergonic2. However, it has not been shown that a single strain can grow on formate by catalysing its conversion to bicarbonate and H2. Here we report that several hyperthermophilic archaea belonging to the Thermococcus genus are capable of formate-oxidizing, H2-producing growth. The actual ΔG values for the formate metabolism are calculated to range between -8 and -20 kJ mol -1 under the physiological conditions where Thermococcus onnurineus strain NA1 are grown. Furthermore, we detected ATP synthesis in the presence of formate as a sole energy source. Gene expression profiling and disruption identified the gene cluster encoding formate hydrogen lyase, cation/proton antiporter and formate transporter, which were responsible for the growth of T. onnurineus NA1 on formate. This work shows formate-driven growth by a single microorganism with protons as the electron acceptor, and reports the biochemical basis of this ability.

- 형식: Research article
- 게재일: 2010년 09월 (BRIC 등록일 2010.09.16)
- 연구진: 국내(교신)+국외 연구진 태극기
- 분야: Molecular_Biology, Genetics, Genomics
Traction Microscopy to Identify Force Modulation in Sub-resolution Adhesions
발표: 한상윤 (University of Texas Southwestern Me...)
일자: 2015년 7월 9일 (목) 오후 01시 (한국시간)
언어: 한국어
참석자 접수신청하기

PerkinElmer 스폰서배너광고 안내
   외부 관련 링크
Google scholar (by Sung Gyun Kang)
Pubmed (by Sung Gyun Kang)
강성균 님의 Lab/개인 홈페이지
관련뉴스 1
관련뉴스 2
   BRIC 관련 링크
강성균 님의 한빛사 논문보기
관련분야 한빛사 논문보기
Molecular_Biology  Genetics  Genomics
강성균 님의 관련인물
  김윤재 (한국해양연구원)
  이현숙 (한국해양과학기술원)
  김은숙 (한국해양연구원)
  배승섭 (한국해양연구원)
  이정현 (한국해양과학기술원)
  임재규 (한국해양과학기술원)
프리미엄 Bio일정 Bio일정 프리미엄 안내
7월 첨단의료산업 전문인력 양성교육
7월 첨단의료산업 전문인력 양성교육
날짜: 2015.07.15~30
장소: 대구경북첨단의료산업진흥재단
비임상 생식독성 과정 교육생 모집
비임상 생식독성 과정 교육생 모집
날짜: 2015.07.16~17
장소: 대전 안전성평가연구소
이전페이지로 돌아가기 맨위로 가기

BRIC 홈    BRIC 소개    회원    검색    문의/FAQ    광고
Copyright © BRIC. All rights reserved. Contact member@ibric.org