Minute™ Lipid Raft Isolation Kit for Mammalian Cells/Tissues
Lipid rafts are small plasma membrane domains containing high level of cholesterol and sphingolipids. Lipid rafts have been implicated in numerous cellular processes including but not limited to signal transduction, membrane trafficking and protein sorting. Lipid-modified proteins and some transmembrane proteins are concentrated in the rafts while other proteins are excluded. Lipid rafts are also found to be associated with Na+/K+ ATPase on plasma membrane.
Lipid rafts are traditionally isolated by sucrose-gradient or OptiPrep gradient using ultracentrifugation that requires large amount of starting material. The protocol is tedious and time consuming. This kit was developed using our proprietary spin-column-based technologies, offering a rapid and easy way to isolate lipid rafts. Total membrane fraction of the sample is first isolated and treated with a non-ionic detergent containing buffer, followed by isolation of detergent resistant fraction by flotation
centrifugation using just a table top microcentrifuge. Highly enriched lipid rafts can be obtained from cultured cells/tissues in less than 90 min without using density gradient and ultracentrifugation.