[DEBUG-WINDOW 처리영역 보기]
즐겨찾기  |  뉴스레터  |  오늘의 정보  |  e브릭몰e브릭몰 회원가입   로그인
BRIC홈 Bio마켓
랩박스 - Neuroscience
스폰서배너광고 안내  배너1
전체보기 할인행사 시약벤더 제품평가 기업정보 BRIC인증마크
 
조회 225  인쇄하기 주소복사 트위터 공유 페이스북 공유 
[엘바이오시스템] [독일 IBA] Detection of Strep-tag® fusion proteins
제품(기술)분류: 실험대행기술 > 기타
전화: 031-229-3350
메일: lbs_reagents(at).daum.net
팩스: 031-229-3351

확대보기
상세정보 사용후기 회사정보 |  등록제품 |  시약벤더 |  진행중 이벤트
  카탈로그 NO :
  제품 생산일 : .
  원산지 :
  생산기업 :
  특징 및 규격  

Detection of Strep-tag® fusion proteins

Two different detection systems are provided:

  1. detection via Strep-Tactin® conjugates (for Strep-/Twin-Strep-tag® proteins)
  2. detection via monoclonal antibodies against Strep-/Twin-Strep-tag® or His-tag

Read which detection system is suitable for your applications:

       Western blot

For direct detection of Strep-tag® fusion proteins in Western blots

  • via chromogenic and/or chemiluminescence reaction (no secondary antibody needed!) use
    - Strep-Tactin
    ® HRP conjugate (HRP, horseradish peroxidase)
    - StrepMAB-Classic HRP conjugate

  • via chromogenic reaction
    - Strep-Tactin® AP conjugate (AP, alkaline phosphatase)

    ELISA

    For protein detection in ELISA use

  • StrepMAB-Classic, unconjugated

  • Strep-Tactin® HRP conjugate

    Immunocytochemistry/Immunohistochemistry

    Visualize your Strep-tag® protein with the following dyes:

    Strep-Tactin® , StrepMAB-Classic and StrepMAB-Immo are available with single fluorescent conjugates at different  wavelengths (488 nm, 546 nm, 645 nm):

  • Strep-tag® fluorescent Chromeo 488 conjugates of Strep-Tactin®, StrepMAB Classic and StrepMAB Immo (50 µg each)

  • Strep-tag® fluorescent Chromeo 546 conjugates of Strep-Tactin®, StrepMAB Classic and StrepMAB Immo (50 µg each)

  • Strep-tag® fluorescent Oyster 645 conjugates of Strep-Tactin®

    (50 µg)

    FACS

    For staining (Twin-)-Strep-tagged proteins in FACS we recommend to use our Strep-Tactin® PE 6-5001-005 or Strep-Tactin® APC 6-5011-005 conjugates, because they lead to a more intensive staining compared with our Oyster and Chromeo dyes and are therefore better suited for a light sensitive application as FACS.

    50 μl conjugate are good for staining of approximately 5x107to 1x108 cells.
     
    Use Strep-Tactin PE / APC as follows:

    - Wash 5x106 pre-cooled cells with 10 ml of a biotin-free physiological wash buffer in a 15 ml reaction tube to remove potentially interfering  ingredients (e.g. biotin).

    - Resuspend the cells in 50 μl wash buffer and transfer cells to a reaction vessel suitable for staining, e.g. a 96-well round bottom microtiter plate.

    - Add 5 µl Strep-Tactin®PE (or Strep-Tactin®APC) to the cells and mix thoroughly by gentle pipetting.

    - Incubate for 20 minutes at 4°C in the dark.

    - Wash cells three times (400xg, 2 min) in 200 μl wash buffer.

    - Cells are ready for FACS

    Note: The staining intensity depends on the concentration of tagged protein and in case of unsatisfying results the concentration of conjugate has to be adjusted.

사용후기
작성자*
내용*
만족도*
코리아바이오믹스
제품평가 공지사항
수입 외산제품 제품평가를 시작합니다.
할인행사 광고 할인행사 광고
에펜도르프코리아
에펜도르프코리아 연구실 필수템 에펜도로프 피펫 원심분리기 PCR장비 세포배양기 특별 프로모션
위로가기
Bio마켓 홈  |  Bio마켓FAQ  |  Bio마켓 문의 및 제안
 |  BRIC소개  |  이용안내  |  이용약관  |  개인정보처리방침  |  이메일무단수집거부
Copyright © BRIC. All rights reserved.  |  문의 member@ibric.org
트위터 트위터    페이스북 페이스북   유튜브 유튜브    RSS서비스 RSS
에펜도르프코리아